E-cigarette use results in suppression of immune and inflammatory-response genes in nasal epithelial cells similar to cigarette smoke
E-cigarette use results in suppression of immune and inflammatory-response genes in nasal epithelial cells similar to cigarette smoke
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DOI：
10.1152/ajplung.00170.2016
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发表时间：
2016-07-01
影响因子：
4.9
通讯作者：
Jaspers, Ilona
中科院分区：
文献类型：
--
作者：
Martin, Elizabeth M.;Clapp, Phillip W.;Jaspers, Ilona
电子烟的使用导致鼻上皮细胞中免疫和炎症反应基因的抑制，这与吸烟相似。[J] .中国生物医学工程学报，2016,31(4):557 - 557。2016年6月10日首次发布；doi: 10.1152 / ajplung.00170.2016。-已知暴露于香烟烟雾会导致宿主防御反应受损和免疫抑制作用。然而，新兴烟草产品（如电子烟）对呼吸道上皮免疫状态的影响在很大程度上是未知的。我们进行了一项临床研究，收集了非吸烟者、吸烟者和电子烟使用者的浅表鼻刮刮活检、鼻腔灌洗、尿液和血清，并评估了他们免疫基因表达谱的变化。根据吸烟史和3- 4周吸烟日记确定吸烟状况，并通过血清可替宁和尿液4-（甲基亚硝胺）-1-（3-吡啶基）-1-丁醇（NNAL）水平进行确认。使用nCounter Human Immunology v2 Expression panel分析鼻刮活检的总RNA。吸烟或吸电子烟导致免疫相关基因的表达减少。吸烟者（n = 53）中表达减少的所有基因在电子烟吸烟者中也表达减少。此外，吸电子烟与大量独特基因的抑制有关（n = 305）。此外，电子烟使用者表现出与吸烟者相同的基因更大的抑制。这对于转录因子表达的抑制尤其明显，如EGR1，它在功能上与吸烟者的5个靶基因表达减少和电子烟使用者的18个靶基因表达减少有关。综上所述，这些数据表明，吸电子烟与大量免疫相关基因的表达下降有关，这与鼻黏膜水平的免疫抑制是一致的。
E-cigarette use results in suppression of immune and inflammatory-response genes in nasal epithelial cells similar to cigarette smoke. Am J Physiol Lung Cell Mol Physiol 311:L135-L144, 2016. First published June 10, 2016; doi:10.1152/ajplung.00170.2016.-Exposure to cigarette smoke is known to result in impaired host defense responses and immune suppressive effects. However, the effects of new and emerging tobacco products, such as e-cigarettes, on the immune status of the respiratory epithelium are largely unknown. We conducted a clinical study collecting superficial nasal scrape biopsies, nasal lavage, urine, and serum from nonsmokers, cigarette smokers, and e-cigarette users and assessed them for changes in immune gene expression profiles. Smoking status was determined based on a smoking history and a 3- to 4-wk smoking diary and confirmed using serum cotinine and urine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) levels. Total RNA from nasal scrape biopsies was analyzed using the nCounter Human Immunology v2 Expression panel. Smoking cigarettes or vaping e-cigarettes resulted in decreased expression of immune-related genes. All genes with decreased expression in cigarette smokers (n = 53) were also decreased in e-cigarette smokers. Additionally, vaping e-cigarettes was associated with suppression of a large number of unique genes (n = 305). Furthermore, the e-cigarette users showed a greater suppression of genes common with those changed in cigarette smokers. This was particularly apparent for suppressed expression of transcription factors, such as EGR1, which was functionally associated with decreased expression of 5 target genes in cigarette smokers and 18 target genes in e-cigarette users. Taken together, these data indicate that vaping e-cigarettes is associated with decreased expression of a large number of immune-related genes, which are consistent with immune suppression at the level of the nasal mucosa.