Zonal expression and activity of glutathione S-transferase enzymes in the mouse olfactory mucosa.
Zonal expression and activity of glutathione S-transferase enzymes in the mouse olfactory mucosa.
复制标题
小鼠嗅粘膜中谷胱甘肽 S-转移酶的区域表达和活性。
DOI：
10.1016/j.brainres.2003.09.012
复制
发表时间：
2004
期刊：
影响因子：
2.9
通讯作者：
Walters,Eric
中科院分区：
文献类型：
--
作者：
Whitby-Logan,GwendolynK;Weech,Michelle;Walters,Eric
啮齿类动物的嗅粘膜的特点是镶嵌的基因表达，表现在各种细胞类型。这些动物的嗅觉敏感性是通过气味受体家族传递的，这些家族在嗅觉神经元群体的各个子集中有明显的表达。表达特定类别的气味受体的受体神经元表现出双侧对称的区域，这通常定义了它们在鼻腔内的位置。特征较少的是嗅觉粘膜的非神经元细胞类型之间的蛋白质的区域表达谱。在这项研究中，我们调查的谷胱甘肽S-转移酶（GST）同工酶（α，亩，和PI）在小鼠嗅粘膜的表达和特点的亩同工酶的带状表达。免疫组织化学和Western印迹分析的GST亩同工酶显示，外侧嗅鼻甲I，Ib，II，IIb和III显示更大的强度的表达GST亩，相比，背侧和隔区的粘膜。GST α和PI同工酶在成年小鼠的嗅觉组织中不显示任何明显的带状组织。当一般底物1-氯-2-4-二硝基苯（CDNB）被用来评估GST活性的嗅觉组织内，侧鼻甲区域显示出较高水平的活动相比，背侧或隔区。GST亩在产前和出生后早期的嗅觉组织表达的分析也揭示了一个区域表达的同工酶。我们将这些发现的意义与代谢地形和嗅觉化学感觉功能联系起来。
The rodent olfactory mucosa is characterized by a mosaic of gene expression that is exhibited among various cell types. Olfactory sensitivity in these animals is conveyed through odorant receptor families that are distinctly expressed within various subsets of the olfactory neuron population. Receptor neurons that express a particular class of odorant receptors exhibit bilaterally symmetric zones, which generally define their location within the nasal cavity. Less characterized are zonal expression profiles of proteins among non-neuronal cell types of the olfactory mucosa. In this study, we survey the expression of three glutathione S-transferase (GST) isozymes (alpha, mu, and pi) in the mouse olfactory mucosa and characterize the zonal expression of the mu isozyme. Immunohistochemistry and Western blot analysis of the GST mu isozyme reveal that the lateral olfactory turbinates I, Ib, II, IIb, and III display a greater intensity of expression for GST mu, in comparison to the dorsal and septal regions of the mucosa. GST alpha and pi isozymes do not display any distinct zonal organization in olfactory tissue of the adult mouse. When the general substrate 1-chloro-2-4-dinitrobenzene (CDNB) was used to assess GST activity within the olfactory tissue, the lateral turbinate regions displayed a higher level of activity when compared to dorsal or septal regions. Analysis of GST mu expression in prenatal and early postnatal olfactory tissue also reveals a zonal expression of the isozyme. We relate the significance of these findings to metabolic topography and olfactory chemosensory function.