CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host.
CryoEM and stability analysis of virus-like particles of potyvirus and ipomovirus infecting a common host.
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DOI：
10.1038/s42003-023-04799-x
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发表时间：
2023-04-19
影响因子：
5.9
通讯作者：
Lopez-Moya, Juan Jose
中科院分区：
文献类型：
--
作者：
Chase, Ornela;Javed, Abid;Byrne, Matthew J.;Thuenemann, Eva C.;Lomonossoff, George P.;Ranson, Neil A.;Lopez-Moya, Juan Jose
甘薯羽毛斑驳病毒（spmv）和甘薯轻度斑驳病毒（SPMMV）是马铃薯病毒科Potyvirus属和ipomomvirus属的成员，以Ipomoea batata为共同宿主，但分别由蚜虫和白蝇传播。家族成员的病毒粒子由围绕RNA基因组的单一外壳蛋白（CP）的多个拷贝的弯曲杆组成。在此，我们报道了在一种复制RNA存在的情况下，通过在烟叶中瞬时表达spmv和SPMMV的CPs来产生病毒样颗粒（VLPs）。通过低温电子显微镜对纯化的VLPs进行分析，得到了分辨率分别为2.6和3.0 Å的结构，显示出类似的左旋螺旋排列，每转8.8个CP亚基，c端位于内表面，并为封装的ssRNA提供了一个结合袋。尽管它们的结构相似，但热稳定性研究表明SPMMV VLPs比SPFMV更稳定。甘薯羽毛斑驳病毒和甘薯轻度斑驳病毒的低温电镜结构揭示了马铃薯病毒科两种不同属病毒的外壳蛋白结构和颗粒结构的守恒性。
Sweet potato feathery mottle virus (SPFMV) and Sweet potato mild mottle virus (SPMMV) are members of the genera Potyvirus and Ipomovirus, family Potyviridae, sharing Ipomoea batatas as common host, but transmitted, respectively, by aphids and whiteflies. Virions of family members consist of flexuous rods with multiple copies of a single coat protein (CP) surrounding the RNA genome. Here we report the generation of virus-like particles (VLPs) by transient expression of the CPs of SPFMV and SPMMV in the presence of a replicating RNA in Nicotiana benthamiana. Analysis of the purified VLPs by cryo-electron microscopy, gave structures with resolutions of 2.6 and 3.0 Å, respectively, showing a similar left-handed helical arrangement of 8.8 CP subunits per turn with the C-terminus at the inner surface and a binding pocket for the encapsidated ssRNA. Despite their similar architecture, thermal stability studies reveal that SPMMV VLPs are more stable than those of SPFMV. CryoEM structures for Sweet potato feathery mottle virus and Sweet potato mild mottle virus reveal conservation of coat protein structure and particle architecture between viruses belonging to two different genera within the family Potyviridae.
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DOI：
10.1073/pnas.95.22.13079
发表时间：
1998-10-27
影响因子：
11.1
作者：
Anandalakshmi, R;Pruss, GJ;Vance, VB
通讯作者：
Vance, VB
影响因子：
--
作者：
Charon, Justine;Theil, Sebastien;Michon, Thierry
通讯作者：
Michon, Thierry
影响因子：
5.4
作者：
Gallo, Araiz;Valli, Adrian;Antonio Garcia, Juan
通讯作者：
Antonio Garcia, Juan
影响因子：
6.7
作者：
Giner A;Lakatos L;García-Chapa M;López-Moya JJ;Burgyán J
通讯作者：
Burgyán J
影响因子：
3.8
作者：
Inoue-Nagata, Alice K.;Jordan, Ramon;Wylie, Stephen J.
通讯作者：
Wylie, Stephen J.