Effects of a multilayered DNA/protamine coating on titanium implants on bone responses
Effects of a multilayered DNA/protamine coating on titanium implants on bone responses
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DOI：
10.1002/jbm.a.35679
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发表时间：
2016-06-01
影响因子：
4.9
通讯作者：
Ohkubo, Chikahiro
中科院分区：
文献类型：
--
作者：
Sakurai, Toshitsugu;Yoshinari, Masao;Ohkubo, Chikahiro
牙科钛 (Ti) 种植体上的 DNA 涂层因其成骨特性而备受关注。本研究的目的是通过模拟体液 (SBF) 浸入实验和大鼠磨牙拔牙槽植入实验，评估钛种植体上多层 DNA/鱼精蛋白 (D/P) 涂层的体外和体内生物活性。使用两种类型的 DNA：300 碱基对 (bp) 和 7000 bp 片段。首先使用三氟乙磺酰氯激活方法将鱼精蛋白固定在钛植入物上，然后通过逐层技术固定鱼精蛋白后交替沉积DNA和鱼精蛋白。通过 X 射线光电子能谱和傅里叶变换红外光谱测量证实了多层 D/P 涂层。在 SBF 浸泡实验中，多层 D/P 涂层钛植入物表面上的磷灰石沉积比未经处理的钛植入物表面沉积得更多。动物植入实验表明，多层 D/P 涂层钛种植体在植入 3 周后提供了显着更高的骨与种植体 (BIC) 接触比。植入后9周的BIC比率没有观察到显着差异。本研究的结果表明，多层 D/P 涂层可促进骨愈合过程早期阶段的新骨形成。 (c) 2016 年 Wiley periodicals, Inc. J Biomed Mater Res Part A：104A：1500-1509，2016 年。
DNA coating on dental titanium (Ti) implants is attracting attention due to its osteogenic properties. The aim of the present study was to evaluate in vitro and in vivo bioactivities of a multilayered DNA/protamine (D/P) coating on Ti implant by simulated body fluid (SBF) immersion experiments and implantation experiments into extracted sockets of rat molars. Two types of DNA, 300 base pair (bp) and 7000 bp fragments, were used. Protamine was initially immobilized onto Ti implants using a tresyl chloride-activated method and DNA and protamine were then alternatively deposited after the immobilization of protamine by a layer-by-layer technique. A multilayered D/P-coating was confirmed by X-ray photoelectron spectroscopy and Fourier-transform infrared spectroscopy measurements. The deposition of apatite progressed more on the surfaces of multilayered D/P-coated Ti implants than on those of nontreated Ti implants in SBF immersion experiments. Animal implantation experiments showed that multilayered D/P-coated Ti implants provided a significantly higher bone-to-implant (BIC) contact ratio 3 weeks after implantation. No significant difference was observed in the BIC ratio 9 weeks after implantation. The results of the present study demonstrated that a multilayered D/P-coating promoted new bone formation at the early stages of the bone healing process. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1500-1509, 2016.