Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients.
Role of adherence in the pathogenesis of Pseudomonas aeruginosa lung infection in cystic fibrosis patients.
复制标题
依从性在囊性纤维化患者铜绿假单胞菌肺部感染发病机制中的作用。
DOI：
10.1128/iai.30.3.694-699.1980
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
Straus,DC
中科院分区：
文献类型：
--
作者：
Woods,DE;Bass,JA;JohansonJr,WG;Straus,DC
铜绿假单胞菌上呼吸道上皮细胞的体外黏附与其在呼吸道的定植有一定的相关性。本研究对20例囊性纤维化患者和20例年龄匹配的对照组进行了检测。所有CF患者除无一例外，均为P。铜绿假单胞菌与口腔黏膜上皮细胞的粘附率分别为19.1±1.1个细菌/颊上皮细胞和2.3±0.3个细菌/细胞(P<；0.0 1)。粘液型铜绿假单胞菌与口腔上皮细胞的粘附率明显低于粗糙集落型铜绿假单胞菌(1.8±0.1对2 4.8±0.9，P<；0.001)。这一差异可能解释了一种普遍的观察，即CF患者呼吸道最初的假单胞菌定植是由于粗菌落类型的微生物。我们进一步证明了P的增加。在体外，铜绿假单胞菌的粘附性与细胞表面一种对蛋白酶敏感的糖蛋白--纤维连接蛋白的丧失以及CF患者唾液中蛋白水解酶水平的升高直接相关。当通过直接放射免疫结合试验检查时，CF患者的口腔细胞只有来自对照组的类似细胞的总细胞表面纤维连接蛋白的17%。从125I标记的不溶纤维蛋白基质中释放125I来测量唾液中的蛋白水解酶水平，在CF患者中大约是对照组的三倍。因此，呼吸道的定植是BYP。铜绿假单胞菌患者与这种生物的口腔细胞粘附性有很好的相关性；粘附性增加与呼吸道上皮细胞表面纤维连接蛋白的减少和唾液蛋白水解酶水平的增加有关。
A correlation has been demonstrated between the in vitro adherence ofPseudomonas aeruginosato upper respiratory tract epithelium and colonization of the respiratory tract by this organism. Twenty patients with cystic fibrosis (CF) and 20 age-matched controls were examined in this study. All of the CF patients but none of the controls were colonized withP. aeruginosaat the time of study.P. aeruginosaadherence to isolated epithelial cells, as determined by an in vitro assay, was 19.1 ± 1.1 bacteria per buccal epithelial cell in the CF patients and 2.3 ± 0.3 bacteria per cell in the controls (P< 0.01).P. aeruginosastrains of the mucoid colony type adhered in significantly lower numbers to buccal epithelial cells than did strains of the rough colony type (1.8 + 0.1 versus 24.8 ± 0.9,P< 0.001). This difference might explain the common observation that the initial pseudomonas colonization of the respiratory tract of CF patients is due to organisms of the rough colony type. We have further demonstrated that increasedP. aeruginosaadherence in vitro varies directly with the loss of a protease-sensitive glycoprotein, fibronectin, from the cell surface, as well as increased levels of salivary proteases in CF patients. When examined by a direct radioimmune binding assay, buccal cells from CF patients possessed only 17% of the total cell surface fibronectin present on similar cells obtained from controls. Salivary protease levels, as measured by125I release from an125I-labeled insoluble fibrin matrix, were increased about threefold in CF patients versus controls. Thus, colonization of the respiratory tract byP. aeruginosain CF patients correlates well with buccal cell adherence of this organism; increased adherence is associated with decreased amounts of fibronectin on respiratory epithelial cell surfaces and increased levels of salivary proteases.