Effects of hypoxia and intracellular iron chelation on hypoxia-inducible factor-1α and -1β in the rat carotid body and glomus cells
Effects of hypoxia and intracellular iron chelation on hypoxia-inducible factor-1α and -1β in the rat carotid body and glomus cells
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DOI：
10.1007/s00418-003-0588-2
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发表时间：
2003-11-01
影响因子：
2.3
通讯作者：
Lahiri, S
中科院分区：
文献类型：
--
作者：
Baby, SM;Roy, A;Lahiri, S
本研究首次提供了关于常氧 (Nx) 中缺氧诱导因子（HIF-1α 和 HIF-1β 蛋白）的发生及其与大鼠颈动脉体 (CB) 球细胞中缺氧 (Hx) 和细胞内 Fe2+ 螯合的相互作用的明确信息。根据免疫组织化学和免疫荧光鉴定与易位到细胞核中的 HIF-1β 结合的 HIF-1α。在 Nx 中，在 CB 血管球细胞中观察到 HIF-1α 的弱表达。然而，将 CB 和血管球细胞暴露于 Hx（Po(2) 类似于或等于 7 Torr）和带有环吡酮乙醇胺（CPX，5 μM）的 Nx 1 小时显示出显着的（P
The present investigation provides for the first time, unambiguous information on the occurrence of hypoxia-inducible factors (HIF-1alpha and HIF-1beta proteins) in normoxia (Nx) and their interaction with hypoxia (Hx) and intracellular Fe2+ chelation in the rat carotid body (CB) glomus cells. HIF-1alpha bound to HIF-1beta translocated into the nucleus is identified on the basis of immunohistochemistry and immunofluorescence. In Nx, a weak expression of HIF-1alpha was observed in CB glomus cells. However, exposure of CB and glomus cells to Hx (Po(2)similar or equal to7 Torr) and Nx with ciclopirox olamine (CPX, 5 muM) for 1 h showed a significant (P