Effects of Aloe Vera Gel Extract in Doped Hydroxyapatite-Coated Titanium Implants on in Vivo and in Vitro Biological Properties
Effects of Aloe Vera Gel Extract in Doped Hydroxyapatite-Coated Titanium Implants on in Vivo and in Vitro Biological Properties
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DOI：
10.1021/acsabm.9b00077
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发表时间：
2019-08-19
影响因子：
4.7
通讯作者：
Bose, Susmita
中科院分区：
文献类型：
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作者：
Banerjee, Dishary;Bose, Susmita
羟基磷灰石涂层钛合金由于羟基磷灰石与天然骨的成分相似，已成为承重应用中骨植入物的流行选择。羟基磷灰石（HA）涂层所表现出的有限的骨诱导特性导致了生长因子或掺杂剂的掺入，以改善骨整合。本研究的目的是调查一种天然存在的芦荟凝胶提取物，醋甘露聚糖，在掺杂羟基磷灰石涂层对体外成骨细胞的活力和在体内新骨形成在大鼠股骨远端模型的影响。通过感应等离子喷涂法在钛合金上制备了银氧化物和二氧化硅掺杂的羟基磷灰石涂层，以分别引入抗菌性能沿着诱导血管生成性能。掺杂涂层进一步连续浸涂乙酰甘露聚糖，以分析其对体内早期骨整合的影响，并连续浸涂壳聚糖，以控制乙酰甘露聚糖从磷酸钙基质中的突释。结果显示醋甘露聚糖从壳聚糖涂层中的受控释放，通过在体外掺入醋甘露聚糖增强了成骨细胞的活力。在植入后5周，通过醋甘露聚糖和壳聚糖涂层在体内观察到具有无缝植入物界面的骨整合改善和新骨形成改善。我们的研究结果表明，天然药物和天然存在的聚合物的组合在掺杂羟基磷灰石涂层钛植入物的骨组织再生承重骨科应用的疗效。
Hydroxyapatite-coated titanium alloys have been a popular choice as bone implants for load-bearing applications for the compositional similarity of hydroxyapatite to natural bone. The limited osteoinductive properties exhibited by the hydroxyapatite (HA) coatings have led to the incorporation of growth factor or dopants for improved osseointegration. This study aims to investigate the effects of a naturally occurring aloe vera gel extract, acemannan, in doped hydroxyapatite coatings on the in vitro osteoblast cell viability and in vivo new bone formation in a rat distal femur model. Silver oxide and silica-doped hydroxyapatite coatings were developed by the induction plasma spray coating method on Ti alloys to introduce antibacterial properties along with induction of angiogenic properties, respectively. The doped coating was further consecutively dip coated with acemannan to analyze its effects on the in vivo early stage osseointegration and chitosan to control the burst release of the acemannan from the calcium phosphate matrix. The results show controlled release of acemannan from the chitosan coatings, with enhanced osteoblast cell viability by the incorporation of acemannan in vitro. Improved osseointegration with a seamless implant interface and improved new bone formation was noted by the acemannan and chitosan coating in vivo, 5 weeks after implantation. Our results demonstrate the efficacy of a combination of natural medicine and naturally occurring polymer in a doped hydroxyapatite-coated titanium implant on the bone tissue regeneration for load-bearing orthopedic applications.