Serotonin and substance P coexist i, neurons of the rat's central nervous system.
Serotonin and substance P coexist i, neurons of the rat's central nervous system.
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血清素和 P 物质在大鼠中枢神经系统的神经元中共存。
DOI：
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发表时间：
1978
影响因子：
11.1
通讯作者：
S. Palay
中科院分区：
文献类型：
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作者：
V. Chan‐Palay;G. Jonsson;S. Palay
用放射自显影、内源性5-羟色胺的荧光组织化学及激发和发射光谱的显微荧光分析等方法，对大鼠延髓中缝和舌下束间细胞群内含5-羟色胺(5-羟色胺)神经元进行了鉴定。用免疫细胞化学方法(未标记的一抗-过氧化物酶、抗过氧化物酶和间接免疫荧光法)和P物质的抗血清对这些延髓神经元的免疫反应进行定位。结果表明，中缝核团和舌下神经束间核是一个异质细胞群，它包含：(1)对5-羟基[(3)H]色胺具有摄取-存储能力的神经元，以及具有与5-羟色胺荧光体相同光谱特征的甲醛诱导荧光的神经元。这些细胞表现出从高到低的荧光强度，没有检测到P物质样免疫反应。(Ii)具有不同的5-羟色胺荧光强度和强至低程度的P物质样免疫反应的神经元。(Iii)具有不同程度P物质样免疫反应的神经元，但没有检测到5-羟色胺荧光或5-羟基[(3)H]色胺摄取和储存能力。这些结果表明，一些神经元只含有或高或低水平的5-羟色胺或P物质，而另一些神经元同时含有不同比例的5-羟色胺和P物质。目前的发现表明，在哺乳动物中枢神经系统的同一神经元中，存在两种假定的递质，生物胺和多肽。
5-Hydroxytryptamine (serotonin)-containing neurons in the rat's medullary raphe and interfascicularis hypoglossi cell groups were identified by means of autoradiography following prolonged intraventricular administration of 5-hydroxy[(3)H]tryptamine, fluorescence histochemistry for the demonstration of endogenous 5-hydroxytryptamine, and microspectrofluorimetric analysis of excitation and emission spectra. Immunocytochemical methods (the unlabeled primary antibody-peroxidase antiperoxidase and indirect immunofluorescence methods) were applied with antisera to substance P in order to localize immunoreactivity in these medullary neurons. It was demonstrated that the raphe nuclei and the interfascicularis hypoglossi nucleus are heterogeneous cell groups that contain: (i) Neurons that display both an uptake-storage capacity for 5-hydroxy[(3)H]tryptamine and a formaldehyde-induced fluorescence with spectral characteristics identical to those of the 5-hydroxytryptamine fluorophor. These cells exhibit high to low fluorescence intensities without detectable substance P-like immunoreactivity. (ii) Neurons with various 5-hydroxytryptamine fluorescence intensities and intense to low degrees of substance P-like immunoreactivity. (iii) Neurons with various degrees of substance P-like immunoreactivity without detectable 5-hydroxytryptamine fluorescence or 5-hydroxy[(3)H]tryptamine uptake and storage capacity. These results indicate that some neurons contain high or low levels of only 5-hydroxytryptamine or substance P, whereas other neurons contain both 5-hydroxytryptamine and substance P in various proportions. The present findings demonstrate the presence of two putative transmitters, a biogenic amine and a polypeptide, within the same neuron in the mammalian central nervous system.