Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms.
Mode-of-Action of Antimicrobial Peptides: Membrane Disruption vs. Intracellular Mechanisms.
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DOI：
10.3389/fmedt.2020.610997
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发表时间：
2020
影响因子：
--
通讯作者：
Henriques ST
中科院分区：
文献类型：
--
作者：
Benfield AH;Henriques ST
抗菌肽是传统抗生素的一种有吸引力的替代品，这是由于它们的物理化学性质、对广谱细菌的活性以及与当前抗生素所使用的那些不同的作用模式。一般来说，抗微生物肽通过破坏细菌的膜或通过进入细菌细胞内与细胞内组分相互作用来杀死细菌。它们的作用模式的表征对于提高它们的活性、避免细菌病原体的耐药性以及加速它们作为治疗剂的使用至关重要。在这里，我们回顾了实验生物物理工具，可以采用模型膜和细菌细胞的抗菌肽的作用模式的特点。
Antimicrobial peptides are an attractive alternative to traditional antibiotics, due to their physicochemical properties, activity toward a broad spectrum of bacteria, and mode-of-actions distinct from those used by current antibiotics. In general, antimicrobial peptides kill bacteria by either disrupting their membrane, or by entering inside bacterial cells to interact with intracellular components. Characterization of their mode-of-action is essential to improve their activity, avoid resistance in bacterial pathogens, and accelerate their use as therapeutics. Here we review experimental biophysical tools that can be employed with model membranes and bacterial cells to characterize the mode-of-action of antimicrobial peptides.