HNF-6 is expressed in endoderm derivatives and nervous system of the mouse embryo and participates to the cross-regulatory network of liver-enriched transcription factors
HNF-6 is expressed in endoderm derivatives and nervous system of the mouse embryo and participates to the cross-regulatory network of liver-enriched transcription factors
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DOI：
10.1006/dbio.1997.8757
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发表时间：
1997-12-15
影响因子：
2.7
通讯作者：
Rousseau, GG
中科院分区：
文献类型：
--
作者：
Landry, C;Clotman, F;Rousseau, GG
肝细胞核因子-6 （HNF-6）是一种富含肝脏的转录因子，它包含一个单一的剪切结构域和一种新型的同源结构域。我们研究了HNF-6在小鼠体内的发育表达模式。原位杂交实验表明，在胚胎日(E) 9，即肝脏分化开始时，肝脏中检测到HNF-6 mRNA。肝脏中HNF-6 mRNA在E12.5 ~ E15期间短暂消失。在转染实验中，HNF-6刺激了HNF-4和HNF-3 β的表达，这两种转录因子已知参与肝脏发育和分化。从E10.5开始，在胰腺中检测到HNF-6，但仅限于外分泌细胞。在发育中的神经系统中也检测到HNF-6。在有丝分裂后神经母细胞中，脑和脊髓在E9-9.5时开始表达HNF-6。后来，HNF-6被限制在脑核、视网膜、脊髓前角和背根神经节。我们观察到HNF-6有助于控制转录因子的表达，并在肝脏、胰腺和神经元分化的早期阶段表达，这表明HNF-6调节了几种发育程序。(C) 1997学术出版社。
Hepatocyte nuclear factor-6 (HNF-6) is a liver-enriched transcription factor that contains a single cut domain and a novel type of homeodomain. Here we have studied the developmental expression pattern of HNF-6 in the mouse. In situ hybridization experiments showed that HNF-6 mRNA is detected in the liver at embryonic day (E) 9, at the onset of liver differentiation. HNF-6 mRNA disappeared transiently from the liver between E12.5 and E15. In transfection experiments HNF-6 stimulated the expression of HNF-4 and of HNF-3 beta, two transcription factors known to be involved in liver development and differentiation. HNF-6 was detected in the pancreas from E10.5 onward, where it was restricted to the exocrine cells. HNF-6 was also detected in the developing nervous system. Both the brain and the spinal cord started to express HNF-6 at E9-9.5 in postmitotic neuroblasts. Later on, HNF-6 was restricted to brain nuclei, to the retina, to the ventral horn of the spinal cord, and to dorsal root ganglia. Our observations that HNF-6 contributes to the control of the expression of transcription factors and is expressed at early stages of liver, pancreas, and neuronal differentiation suggest that HNF-6 regulates several developmental programs. (C) 1997 Academic Press.