Humanin levels in human seminal plasma and spermatozoa are related to sperm quality.
Humanin levels in human seminal plasma and spermatozoa are related to sperm quality.
复制标题
人类精浆和精子中的护脑素水平与精子质量有关。
作者：
Rao Meng;Wu Zhao;Wen Ya;Wang Rui;Zhao Shuhua;Tang Li
目的探讨人蛋白在人精浆中的表达及其与精子质量的关系。材料与方法收集164例男性门诊患者精浆标本，进行人蛋白/精子人蛋白水平的测定。用免疫荧光法检测人蛋白在精子中的定位，用双抗体夹心法测定精子表面活性物质(SP)/精子蛋白水平。结果人蛋白在精子的中段有表达。SP中人的浓度范围为24.4~285.1微克/毫升，中位数为89.7微克/毫升。正常精子症患者血浆P物质含量显著高于少精子症患者(p<；0.001)、弱精子症患者(p=0.002)和少弱精子症患者(p<；0.001)。Spearman相关分析显示，SP人蛋白浓度与精子密度(r=0.75，p<；=0.001)和进行性精子活动率(r=0.29，p<；；0.001)呈显著正相关。精子人蛋白水平与进行性精子活动率呈显著正相关(r=0.70，p<；0.001)。此外，上游精子中人蛋白水平显著高于非上游精子(p=0.03)。结论精浆和精子人蛋白水平与精子质量呈显著正相关，尤其与精子活力呈显著正相关。SP人蛋白的来源及其在精子发生中的作用有待进一步研究。
BackgroundHumanin has reportedly been expressed in testis and spermatozoa, but no study has yet reported its presence in human seminal plasma (SP).ObjectiveThe aim of this study was to investigate the presence of humanin in human SP and to determine the correlation between humanin levels in SP/spermatozoa and sperm quality.Materials and methodsSemen samples for SP/sperm humanin level measurement were collected from 164 patients who attended our andrology clinic for fertility evaluation. The localization of humanin in spermatozoa was evaluated using an immunofluorescence method, and SP/sperm humanin levels were measured with ELISA. Correlations between SP/sperm humanin levels and sperm parameters were analyzed.ResultsHumanin was expressed in the midpiece of the spermatozoa. Humanin concentrations in the SP ranged from 24.4 to 285.1 pg/mL, with a median of 89.7 pg/mL. The SP humanin concentrations in patients with normospermia were significantly higher than those in patients with oligospermia (p< 0.001), asthenospermia (p= 0.002), and oligoasthenospermia (p< 0.001). Spearman analysis showed a positive and significant correlation between SP humanin concentration and sperm concentration (r= 0.75,p< 0.001), and progressive sperm motility (r= 0.29,p< 0.001). Sperm humanin level was significantly and positively associated with progressive sperm motility (r= 0.70,p< 0.001). In addition, a significantly higher level of humanin was found in swim‐up spermatozoa than in non‐swim‐up spermatozoa (p= 0.03).ConclusionsSeminal plasma and sperm humanin levels were significantly and positively correlated with sperm quality, especially sperm motility. Further studies of the origin of SP humanin and its role in spermatogenesis should be conducted.