Mass production and dynamic imaging of fluorescent nanodiamonds
Mass production and dynamic imaging of fluorescent nanodiamonds
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DOI：
10.1038/nnano.2008.99
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发表时间：
2008-05-01
影响因子：
38.3
通讯作者：
Fann, Wunshain
中科院分区：
文献类型：
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作者：
Chang, Yi-Ren;Lee, Hsu-Yang;Fann, Wunshain
荧光纳米金刚石是一种新型纳米材料，具有多种有用的特性，包括良好的生物相容性（1）、优异的光稳定性（1,2）以及易于表面功能化（2,3）。此外，当被激光激发时，纳米金刚石内的缺陷中心会发射能够穿透组织的光子，这使其非常适合生物成像应用（1,2,4）。在此，我们表明通过用氦离子照射合成的金刚石纳米微晶可以大量生产出明亮的荧光纳米金刚石。这种荧光足够明亮和稳定，能够通过单光子或双光子激发荧光显微镜对细胞内的单个粒子进行三维追踪。对于小至25纳米的粒子，其优异的光物理特性仍然保持，这表明荧光纳米金刚石是一种用于体内长期追踪和成像的理想探针，具有良好的时间和空间分辨率。
Fluorescent nanodiamond is a new nanomaterial that possesses several useful properties, including good biocompatibility(1), excellent photostability(1,2) and facile surface functionalizability(2,3). Moreover, when excited by a laser, defect centres within the nanodiamond emit photons that are capable of penetrating tissue, making them well suited for biological imaging applications(1,2,4). Here, we show that bright fluorescent nanodiamonds can be produced in large quantities by irradiating synthetic diamond nanocrystallites with helium ions. The fluorescence is sufficiently bright and stable to allow three-dimensional tracking of a single particle within the cell by means of either one- or two-photon-excited fluorescence microscopy. The excellent photophysical characteristics are maintained for particles as small as 25 nm, suggesting that fluorescent nanodiamond is an ideal probe for long-term tracking and imaging in vivo, with good temporal and spatial resolution.