EVALUATION OF METHODS USED IN COUNTING AZOTOBACTERS

EVALUATION OF METHODS USED IN COUNTING AZOTOBACTERS
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DOI:
10.1111/j.1365-2672.1968.tb00367.x
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发表时间:
1968-01-01
期刊:
JOURNAL OF APPLIED BACTERIOLOGY
影响因子:
--
通讯作者:
ISHAC, YZ
ISHAC, YZ
中科院分区:
其他
文献类型:
--
作者:
ABDELMAL.Y;ISHAC, YZ

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偶氮细菌的常用计数方法。在土壤中,菌落计数和MPN,使用由直接显微镜计数确定密度的细胞悬浮液进行比较。在表面板、MPN和总细胞数之间发现了高度显著的正相关,但MPN估计值更接近总细胞数。在液体培养基中加入1 ~ 1.5 g琼脂/l,可促进固氮菌的繁殖,并有助于记录MPN估计中的生长情况。
The common methods of countingAzotobacterspp. in soil, colony counts and MPN, were compared using cell suspensions of densities determined by direct microscopic counts. A highly significant positive correlation was found between the surface‐plate, MPN and total cell counts, but the MPN estimations approximated total cell counts much more closely. Inclusion of 1‐1.5 g of agar/l of the liquid medium enhanced multiplication of azotobacters and facilitated the recording of growth in MPN estimations.