Distribution of calbindin, parvalbumin, and calretinin immunoreactivity in the reticular thalamic nucleus of the marmoset: Evidence for a medial leaflet of incertal neurons
Distribution of calbindin, parvalbumin, and calretinin immunoreactivity in the reticular thalamic nucleus of the marmoset: Evidence for a medial leaflet of incertal neurons
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DOI：
10.1006/exnr.2000.7436
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发表时间：
2000-08-01
影响因子：
5.3
通讯作者：
Goodchild, AK
中科院分区：
文献类型：
--
作者：
FitzGibbon, T;Solomon, SG;Goodchild, AK
网状丘脑核(RTN)位于背侧丘脑和皮质之间，以及网状丘脑投射的抑制性质，这导致了人们认为它是向皮质传递感觉信息流的“门”。新大陆昼夜活动的猴子，绒猴，Callithrix Jacchus正在成为研究感觉处理的重要的“模式灵长类”。为了与其他物种进行比较，我们研究了尼氏染色的胞体和钙结合蛋白、小白蛋白和钙视黄素在丘脑腹侧的分布。用标准免疫组织化学、ExtraAvidin-HRP和二氨基联苯胺反应产物标记细胞。小白蛋白免疫反应细胞群在大小和方向上基本相同。Calbindin和Calretinin免疫反应细胞仅见于紧邻丘脑背侧延髓外板的RTN内侧缘和靠近腹侧RTN的外侧。这些细胞被认为是吨Incerta(Zi)的一部分。根据绒猴Zi对钙结合蛋白的免疫反应性，可将其分为背侧区和腹侧区。Zi核和丘脑底核Luysi核均含有散在分布的Calbindin和Calretinin免疫反应细胞，树突清晰可见。这些细胞与丘脑背侧的细胞明显不同。RTN、ZI和丘脑底核的小白蛋白免疫反应细胞平均比其他钙结合蛋白阳性的神经元大。未来报道RTN的传入和传出投射的研究必须根据RTN和Zi Somata的密切对应来看待它们的结果。(C)2000年学术出版社。
The placement of the reticular thalamic nucleus (RTN) between the dorsal thalamus and the cortex and the inhibitory nature of reticulothalamic projections has led to suggestions that it "gates" the flow of sensory information to the cortex. The New World diurnal monkey, the marmoset, Callithrix jacchus is emerging as an important "model primate" for the study of sensory processing. We have examined the distribution of Nissl-stained somata and calbindin, parvalbumin, and calretinin immunoreactivity in the ventral thalamus for comparison with other species. Cells were labeled using standard immunohistochemistry, ExtraAvidin-HRP, and diaminobenzidine reaction products. The RTN is constituted by a largely homogeneous population of parvalbumin immunoreactive cells with respect to size and orientation. Calbindin and calretinin immunoreactive cells were only found along the medial edge of the RTN adjacent to the external medullary lamina of the dorsal thalamus and laterally near the ventral RTN. These cells were considered to be part of the tons incerta (ZI). The marmoset ZI could be subdivided into dorsal and ventral regions on the basis of its immunoreactivity to calcium binding proteins. Both the ZI and nucleus subthalamicus Luysi contained scattered calbindin and calretinin immunoreactive cells with well-defined dendritic processes. These cells were clearly different to cells in the dorsal thalamus. Parvalbumin immunoreactive cells in RTN, ZI, and subthalamic nucleus were on average larger than neurons positive for the other calcium binding proteins. Future studies reporting the afferent and efferent projections to the RTN must view their results in terms of the close apposition Of RTN and ZI somata. (C) 2000 Academic Press.