Fungiform taste bud degeneration in C57BL/6J mice following chorda-lingual nerve transection
Fungiform taste bud degeneration in C57BL/6J mice following chorda-lingual nerve transection
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DOI：
10.1002/cne.21436
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发表时间：
2007-09-10
影响因子：
2.5
通讯作者：
Hill, David L.
中科院分区：
文献类型：
--
作者：
Guagliardo, Nick A.;Hill, David L.
味蕾的正常形态和功能依赖于神经支配。鼓索神经损伤后菌状味蕾变性在大鼠、仓鼠和沙土鼠中已有很好的记录。本研究观察了成年C57BL/6J小鼠味觉系统完整和单侧舌索神经切断后菌状味蕾的分布和结构。用细胞角蛋白8对菌状味蕾进行观察和测量。对照组小鼠舌尖前部(1 Mm)的味蕾较小，数量较多。神经切断后5天，舌侧味蕾变小，数量减少，15天后味蕾的大小和数目继续减少。退化的菌状味蕾较小，是因为味蕾细胞的丧失，而不是味蕾形态的变化。虽然在切断后15天，更多的真菌状乳头中几乎所有的味蕾都消失了，但与完整的小鼠相比，舌尖前部保留了近一半的味蕾。幸存下来的味蕾不能用剩余完整神经的明显神经支配来解释。神经切断的对侧效应也被观察到；由于味蕾细胞数量的增加，味蕾更大。这些数据首次描述了成年小鼠的菌状味蕾和单侧神经切断后的变性。它们为可以使用基因工程小鼠的更多机械性研究提供了基础。(C)2007年Wiley-Liss，Inc.
Taste buds are dependent on innervation for normal morphology and function. Fungiform taste bud degeneration after chorda tympani nerve injury has been well documented in rats, hamsters, and gerbils. The current study examines fungiform taste bud distribution and structure in adult C57BL/6J mice from both intact taste systems and after unilateral chorda-lingual nerve transection. Fungiform taste buds were visualized and measured with the aid of cytokeratin 8. In control mice, taste buds were smaller and more abundant on the anterior tip (< 1 mm) of the tongue. By 5 days after nerve transection taste buds were smaller and fewer on the side of the tongue ipsilateral to the transection and continued to decrease in both size and number until 15 days posttransection. Degenerating fungiform taste buds were smaller due to a loss of taste bud cells rather than changes in taste bud morphology. While almost all taste buds disappeared in more posterior fungiform papillae by 15 days posttransection, the anterior tip of the tongue retained nearly half of its taste buds compared to intact mice. Surviving taste buds could not be explained by an apparent innervation from the remaining intact nerves. Contralateral effects of nerve transection were also observed; taste buds were larger due to an increase in the number of taste bud cells. These data are the first to characterize adult mouse fungiform taste buds and subsequent degeneration after unilateral nerve transection. They provide the basis for more mechanistic studies in which genetically engineered mice can be used. (c) 2007 Wiley-Liss, Inc.