The chloroplast protein import machinery: a review.
The chloroplast protein import machinery: a review.
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叶绿体蛋白输入机制：综述。
DOI：
10.1007/978-1-59745-466-7_12
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发表时间：
2007
影响因子：
--
通讯作者：
B. Bölter
中科院分区：
文献类型：
--
作者：
Friederike Hörmann;J. Soll;B. Bölter
质体是植物和藻类细胞中普遍存在的一类细胞器。最突出的是叶绿体，它进行光合作用和脂肪酸和氨基酸的生物合成等重要过程。与线粒体一样，叶绿体是由单一的内共生事件产生的。它们被认为是从一种被早期真核生物祖先吞没的古老蓝藻进化而来的。在进化过程中，质体基因组大大减少，大部分基因已转移到宿主细胞核中。因此，超过98%的质体蛋白在细胞质核糖体上翻译。它们必须在翻译后靶向并导入细胞器。靶向是由细胞质蛋白辅助的，细胞质蛋白与运往质体的蛋白相互作用，从而使它们处于进口能力状态。在到达目标细胞器后，许多蛋白质必须克服叶绿体内外膜的屏障。这一过程分别由叶绿体外层（Toc复合体）和内部（Tic复合体）的复杂分子机器介导。大多数进入叶绿体内隔室的蛋白质含有可切割的n端转运肽，而大多数外包膜成分插入膜时没有这种靶向肽。
Plastids are a heterogeneous family of organelles found ubiquitously in plant and algal cells. Most prominent are the chloroplasts, which carry out such essential processes as photosynthesis and the biosynthesis of fatty acids as well as of amino acids. As mitochondria, chloroplasts derived from a single endosymbiotic event. They are believed to have evolved from an ancient cyanobacterium, which was engulfed by an early eukaryotic ancestor. During evolution the plastid genome has been greatly reduced and most of the genes have been transferred to the host nucleus. Consequently, more than 98% of all plastid proteins are translated on cytosolic ribosomes. They have to be posttranslationally targeted to and imported into the organelle. Targeting is assisted by cytosolic proteins, which interact with proteins destined for plastids and thereby keep them in an import-competent state. After reaching the target organelle, many proteins have to conquer the barrier of the chloroplast outer and inner envelopes. This process is mediated by complex molecular machines in the outer (Toc complex) and inner (Tic complex) envelope of chloroplasts, respectively. Most proteins destined for compartments inside the chloroplast contain a cleavable N-terminal transit peptide, whereas most of the outer envelope components insert into the membrane without such a targeting peptide.
DOI：
10.1073/pnas.0607150103
发表时间：
2006-10-24
影响因子：
11.1
作者：
Chigri, Fatima;Hoermann, Friederike;Vothknecht, Ute C.
通讯作者：
Vothknecht, Ute C.
影响因子：
3.3
作者：
Becker, T;Hritz, J;Schleiff, E
通讯作者：
Schleiff, E
影响因子：
11.6
作者：
Duy, Daniela;Wanner, Gerhard;Philippar, Katrin
通讯作者：
Philippar, Katrin