Long-term stability of serum components in the Janus Serum Bank
Long-term stability of serum components in the Janus Serum Bank
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DOI：
10.1080/00365510701809235
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发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Morkrid, Lars
中科院分区：
文献类型：
--
作者：
Gislefoss, Randi E.;Grimsrud, Tom K.;Morkrid, Lars
客观的。生物样本库材料经常用于流行病学研究，但在 225°C 下长期储存血清可能会降低样本质量。了解生物样品中成分的稳定性是解释此类研究的基础。材料和方法。我们研究了在 225°C 下保存 25 年和 2 年的血清样品中七种生物成分的稳定性，并与 1 个月前的样品进行比较。每组 130 名献血者的样本是在随访期间从未诊断出癌症的男性中随机抽取的。我们比较了中位数和均值的分布、离差和局部性，并建立了各成分的参考区间。结果。该研究表明，随着时间的推移，钠、钙、铁和肌酐的水平存在不显着且数值上较小的差异。新鲜样品和 25 年样品之间的尿酸 (27.6%)、钾 (+ 26.4%) 和胆红素 (259.4%) 平均值之间的差异表明，冷冻前的样品处理和长期储存过程中的降解可能会给易损成分带来相当大的偏差。结论。 225°C 保存的血清中，不同血清成分之间的水平稳定性存在很大差异。冷冻前样品处理的差异可能会对易受影响的成分产生偏差。本研究支持在流行病学研究中使用生物样本库材料时仔细匹配病例和控制储存时间的常规做法。
Objective. Biobank material is frequently used in epidemiological studies, but long- term storage of serum at 225 C may reduce the quality of the samples. Knowledge about the stability of components in biological samples is fundamental for the interpretation of such studies. Material and methods. We investigated the stability of seven biological components in serum samples stored at 225 C for 25 and 2 years compared with 1- month- old samples. Specimens from 130 blood donors from each group were randomly selected among men without a cancer diagnosis during the follow- up time. We compared the distribution, dispersion and localization of medians and means, and established reference intervals of the components. Results. The study demonstrated non- significant and numerically small differences in the levels of sodium, calcium, iron and creatinine over time. Differences between mean values for uric acid ( 27.6%), potassium (+ 26.4%) and bilirubin ( 259.4%) between fresh and 25year- old samples indicated that sample handling before freezing and degradation during long- term storage may introduce a considerable bias for vulnerable components. Conclusions. There is large variation in level stability between different serum components in serum stored at 225 C. Differences in sample handling before freezing may introduce bias on vulnerable components. The present study supports a routine of careful matching of cases and controls on storage time in epidemiological studies when biobank material is used.