MECHANISMS OF PROTEOGLYCAN INHIBITION OF HYDROXYAPATITE GROWTH
MECHANISMS OF PROTEOGLYCAN INHIBITION OF HYDROXYAPATITE GROWTH
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DOI：
10.1007/bf02553709
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发表时间：
1985-01-01
影响因子：
4.2
通讯作者：
BOSKEY, AL
中科院分区：
文献类型：
--
作者：
CHEN, CC;BOSKEY, AL
纯化的牛鼻软骨蛋白聚糖（聚集体和含亚基的组分）和生理大小的软骨素4-硫酸盐在较小程度上延迟了体外接种的羟基磷灰石（HA）生长。这些大分子的大流体动力学尺寸和高电荷密度被认为与蛋白聚糖抑制HA形成和生长的能力有关。通过比较软骨素4-硫酸盐及其类似物的抑制能力，以及通过比较用蛋白聚糖聚集体或软骨素4-硫酸盐涂覆的HA晶种与未涂覆晶体的生长，证明了蛋白聚糖的负电荷参与这种抑制。在纯化实验中，纯化的硫酸软骨素是比未处理的HA生长抑制剂效率更低的HA生长抑制剂。类似分子大小的未水解硫酸软骨素。硫酸葡聚糖的抑制效果高于中性葡聚糖。这两个实验都表明，硫酸基团在蛋白多糖调节矿物质沉积中起着重要作用。在涂层实验中，HA晶种与蛋白聚糖聚集体的预涂层作为时间的函数的沉淀的HA的量减少，这表明蛋白聚糖可能会阻止HA表面上的活性成核位点和减缓生长过程。软骨素4-硫酸盐具有类似但较弱的包衣效果。中性葡聚糖对HA几乎没有亲和力，没有影响。
Purified bovine nasal cartilage proteoglycans (aggregate and subunit containing fractions) and to a lesser degree, chondroitin 4-sulfate of physiological size, retard seeded hydroxyapatite (HA) growth in vitro. The large hydrodynamic size and high charge density of these marcromolecules are believed to be associated with the ability of proteoglycans to inhibit HA formation and growth. The involvement of the negative charges of proteoglycans in this inhibition is demonstrated by comparing the inhibitory ability of chondroitin 4-sulfate and its desulfated analog, and by comparing the growth of HA seed crystals coated either with proteoglycan aggregates or chondroitin 4-sulfate to that of uncoated crystals. In the desulfation experiments, desulfated chondroitin sulfate was a less efficient HA growth inhibitor than untreated., undesulfated chondroitin sulfate of similar molecular size. Dextran sulfate showed higher inhibitory effectiveness than unchanged neutral dextran. Both experiments suggest that sulfate groups play an important role in the regulation of mineral deposition by proteoglycans. In the coating experiment, precoating of HA seed crystals with proteoglycan aggregates decreased the amount of HA precipitated as a function of time, suggesting proteoglycans may block the active nucleating sites on HA surface and slow down the growth process. Chondroitin 4-sulfate had a similar but weaker coating effect. Neutral dextran, having little affinity for HA, had no effect.