High levels of CD44 expression distinguish virgin from antigen-primed B cells.
High levels of CD44 expression distinguish virgin from antigen-primed B cells.
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DOI：
10.1084/jem.173.3.763
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发表时间：
1991-03-01
期刊：
影响因子：
--
通讯作者：
Puré E
中科院分区：
文献类型：
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作者：
Camp RL;Kraus TA;Birkeland ML;Puré E
通过表面免疫球蛋白 (Ig) 对 B 细胞进行体外多克隆刺激可在 24 小时内诱导 CD44 蛋白水平大幅增加，而其他刺激（例如脂多糖、佛波醇 12,13 二丁酸酯和白细胞介素 4）无法显着上调 CD44。抗 Ig 处理的 B 淋巴细胞上 CD44 蛋白表达显着增加，与 CD44 特异性 mRNA 的增加相关。使用从三硝基苯基匙孔血蓝蛋白免疫小鼠中分离的 B 细胞进行的细胞分选实验表明，短期抗原特异性、IgG 分泌细胞和长期抗原引发的 B 细胞都完全是 CD44 高的。我们推测表面 Ig 刺激介导的 CD44 表达快速持续增加可能会改变抗原引发的 B 细胞的归巢特性。
The in vitro polyclonal stimulation of B cells through their surface immunoglobulin (Ig) induces substantial increases in CD44 protein levels within 24 hours, whereas other stimuli (e.g., lipopolysaccharide, phorbol 12,13 dibutyrate, and interleukin 4) fail to significantly upregulate CD44. The marked increase in CD44 protein expression on anti-Ig-treated B lymphocytes correlates with an increase in CD44-specific mRNA. Cell sorting experiments with B cells isolated from trinitrophenyl-keyhole limpet hemocyanin-immunized mice demonstrate that both short-term antigen-specific, IgG-secreting cells and long-term antigen-primed B cells are exclusively CD44high. We speculate that the rapid and sustained increase in CD44 expression mediated by surface Ig stimulation may alter the homing properties of antigen-primed B cells.