CHEMICAL COMPOSITION OF WOOL .V. EPICUTICLE
CHEMICAL COMPOSITION OF WOOL .V. EPICUTICLE
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DOI：
10.1071/bi9680375
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发表时间：
1968-01-01
期刊：
影响因子：
--
通讯作者：
BRADBURY, JH
中科院分区：
文献类型：
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作者：
KING, NLR;BRADBURY, JH
分离了氯（上表皮）和溴引起的羊毛表皮膜。它们的厚度，通过Au阴影样品的电子显微镜测定，分别为32 [±] 10和140 [±] 40。上表皮由78%的蛋白质，5%的脂质，4%的灰分，和一个可以忽略不计的量的碳水化合物。对上表皮和填充Allworden囊的溶解蛋白质的氨基酸分析表明，（除甘氨酸外）上表皮中存在的量大于整个表皮中存在的量的那些氨基酸在溶解蛋白质中存在的量较小，反之亦然。此外，上表皮的氨基酸组成更接近于角质层的蛋白质从细胞膜复合物。这一事实，连同其低脂质含量，表明它可能不是来自角质层细胞膜复合物。溴引起的角质层馏分的氨基酸组成是不同的，但同样相似，角质层和上表皮。这个较厚的膜显然是由上表皮加上外表皮的外部部分组成的，正如以前其他工作人员所提出的那样。
The cuticle membranes of wool raised by chlorine (epicuticle) and by bromine were isolated. Their thicknesses, as determined by electron microscopy of Au shadowed specimens, were 32 [plus or minus] 10 A and 140 [plus or minus] 40 A respectively. The epicuticle consisted of 78% protein, 5% lipid, 4% ash, and a negligible amount of carbohydrate. Amino acid analyses of the epicuticle and the dissolved protein which fills the Allworden sacs showed that (with the exception of glycine) those amino acids which were present in larger amount in the epicuticle than in the whole cuticle were present in smaller amount in the dissolved protein and vice versa. Furthermore, the amino acid composition of the epicuticle resembled that of the cuticle more closely than that of the protein from the cell membrane complex. This fact, together with its low lipid content, suggests that it may not originate from the cuticle cell membrane complex. The amino acid composition of the cuticle fraction raised by bromine was different from, but equally similar to, that of cuticle and epicuticle. This thicker membrane apparently consisted of the epicuticle plus the outer section of the exocuticle as previously proposed by other workers.