Designing and optimizing library selection strategies for generating high-affinity antibodies
Designing and optimizing library selection strategies for generating high-affinity antibodies
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DOI：
10.1016/s0167-7799(97)84205-9
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发表时间：
1997-02-01
影响因子：
17.3
通讯作者：
Hoogenboom, HR
中科院分区：
文献类型：
--
作者：
Hoogenboom, HR
自20世纪90年代初发明以来，抗体噬菌体展示已经彻底改变了单克隆抗体的产生及其工程化，现在可以在完全绕过免疫系统的系统中产生与任何选定的靶抗原结合的抗体，而无需使用实验室动物或杂交瘤。从单罐噬菌体文库中制备抗体，并在必要时将其亲和力提高到皮摩尔范围，从未如此简单。在这篇综述中，提出了各种基于噬菌体库的高亲和力抗体分离策略。
Since its invention at the beginning of the 1990s, antibody phage display has revolutionized the generation of monoclonal antibodies and their engineering, it is now possible to create antibodies binding to any chosen target antigen without the use of laboratory animals or hybridomas, in a system that completely by-passes the immune system. Making antibodies from single-pot phage libraries, and improving their affinity up to the picomolar range if necessary, has never appeared easier. In this review, a variety of phage library-based strategies for the isolation of high-affinity antibodies are presented.