In vitro and in vivo pathogenicity of Salmonella enteritidis clinical strains isolated from North America
In vitro and in vivo pathogenicity of Salmonella enteritidis clinical strains isolated from North America
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DOI：
10.1007/s00203-011-0719-4
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发表时间：
2011-06
影响因子：
2.8
通讯作者：
D. Shah;Xiaohui Zhou;Tarek Addwebi;M. Davis;D. Call
中科院分区：
文献类型：
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作者：
D. Shah;Xiaohui Zhou;Tarek Addwebi;M. Davis;D. Call
沙门氏菌是全球食源性胃肠炎的主要原因。本研究对48株S.对从北美沙门氏菌病临床病例中分离的沙门氏菌进行了毒力相关性状的检测，包括细胞侵袭性、生物膜、运动性、毒力质粒的存在和经口攻击小鼠的毒力。大多数菌株表现出高侵袭力（n= 45），而只有少数菌株（n= 3）表现出低侵袭力。所有低侵袭性菌株（100%，3/3）均为生物膜阴性，而高侵袭性菌株中生物膜阳性和阴性表型的分布分别为53.4%（24/45）和46.6%（21/45）。体外细胞侵袭性与生物膜形成（Fisher精确检验，P= 0.23）或aspvB基因（毒力相关质粒的标记物）的存在无关（Fisher精确检验，P= 1）。细胞侵袭性和运动性之间没有相关性（斯皮尔曼秩检验，r=-0.15;P= 0.27）。经口攻击小鼠的毒力测试显示，低侵袭性菌株与高侵袭性菌株一样毒力，表明体外细胞侵袭性与体内毒力无关。总之，我们表明，尽管临床菌株之间的表型多样性的S。然而，大多数菌株在体外和体内都具有高度侵袭性。
Salmonella enteritidisis a leading cause of food-borne gastroenteritis worldwide. In this study, 48 strains ofS. enteritidisisolated from clinical cases of salmonellosis in North America were tested for their virulence-associated traits including cell invasiveness, biofilm, motility, presence of a virulence plasmid, and virulence in orally challenged mice. The majority of strains exhibited high invasiveness (n= 45), whereas only few strains (n= 3) exhibited low invasiveness. All low-invasive strains (100%, 3/3) were biofilm negative, whereas the distribution of biofilm positive and negative phenotypes among high-invasive strains was 53.4% (24/45) and 46.6% (21/45), respectively. The in vitro cell invasiveness was not associated with biofilm formation (Fisher’s exact test,P= 0.23) or the presence of aspvBgene, a marker for the virulence-associated plasmid (Fisher’s exact test,P= 1). There was no correlation between cell invasiveness and motility (Spearman’s rank test,r= −0.15;P= 0.27). Virulence testing in orally challenged mice revealed that the low-invasive strains were as virulent as high-invasive strains, indicating that in vitro cell invasiveness did not correlate with in vivo virulence. In conclusion, we show that despite phenotypic diversity among clinical strains ofS. enteritidis, the majority of strains are highly invasive in vitro and in vivo.