The vacuolar protein sorting genes in insects: A comparative genome view
The vacuolar protein sorting genes in insects: A comparative genome view
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昆虫液泡蛋白分选基因：比较基因组视图
DOI：
10.1016/j.ibmb.2014.11.007
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发表时间：
2015-07-01
影响因子：
3.8
通讯作者：
Blissard, Gary
中科院分区：
文献类型：
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作者：
Li, Zhaofei;Blissard, Gary
在真核细胞中，受调控的囊泡运输对于指导蛋白质转运以及膜脂质和蛋白质的回收和降解至关重要。通过仔细调节的运输囊泡，内膜系统执行大量和重要的动态细胞功能，同时保持细胞膜系统的完整性。在酿酒酵母中的遗传研究已经确定了大约50个参与囊泡运输的液泡蛋白分选（VPS）基因，并且这些基因中的大多数也在哺乳动物中具有特征。VPS蛋白形成不同的功能复合物，其包括称为ESCRT、retromer、CORVET、HOPS、CARP和PI 3 K-III的复合物。昆虫中VPS蛋白的直系同源物知之甚少。在这里，与新注释的Manducasexta基因组，我们进行了酵母，人类和13个测序的昆虫基因组中的VPS蛋白的基因组比较分析代表的订单Hydeltera，双翅目，半翅目，Phthiraptera，鳞翅目，鞘翅目。氨基酸序列比对和结构域/基序结构分析表明，ESCRT，retromer，CORVET，HOPS，GARP和P13 K-III的大部分组分在酵母，昆虫和人类中进化保守。然而，与在人类基因组中观察到的VPS基因扩增相反，在6个昆虫目中仅扩增了4个VPS基因（VPS 13、VPS 16、VPS 33和VP 537）。此外，VPS 2仅在来自Phthiraptera、Linguoptera和Coleoptera的物种中扩增。这些研究为了解酵母、昆虫和人类基因组中囊泡运输的演变提供了基线，也为进一步解决VPS蛋白在昆虫中的特定功能作用提供了基础。(C)2014爱思唯尔有限公司版权所有。
In eukaryotic cells, regulated vesicular trafficking is critical for directing protein transport and for recycling and degradation of membrane lipids and proteins. Through carefully regulated transport vesicles, the endomembrane system performs a large and important array of dynamic cellular functions while maintaining the integrity of the cellular membrane system. Genetic studies in yeast Saccharomyces cerevisiae have identified approximately 50 vacuolar protein sorting (VPS) genes involved in vesicle trafficking, and most of these genes are also characterized in mammals. The VPS proteins form distinct functional complexes, which include complexes known as ESCRT, retromer, CORVET, HOPS, CARP, and PI3K-III. Little is known about the orthologs of VPS proteins in insects. Here, with the newly annotated Manduca sexta genome, we carried out genomic comparative analysis of VPS proteins in yeast, humans, and 13 sequenced insect genomes representing the Orders Hymenoptera, Diptera, Hemiptera, Phthiraptera, Lepidoptera, and Coleoptera. Amino acid sequence alignments and domain/motif structure analyses reveal that most of the components of ESCRT, retromer, CORVET, HOPS, GARP, and P13K-III are evolutionarily conserved across yeast, insects, and humans. However, in contrast to the VPS gene expansions observed in the human genome, only four VPS genes (VPS13, VPS16, VPS33, and VP537) were expanded in the six insect Orders. Additionally, VPS2 was expanded only in species from Phthiraptera, Lepidoptera, and Coleoptera. These studies provide a baseline for understanding the evolution of vesicular trafficking across yeast, insect, and human genomes, and also provide a basis for further addressing specific functional roles of VPS proteins in insects. (C) 2014 Elsevier Ltd. All rights reserved.