ELECTRO-PHYSIOLOGICAL PROPERTIES OF INVITRO PURKINJE-CELL DENDRITES IN MAMMALIAN CEREBELLAR SLICES
ELECTRO-PHYSIOLOGICAL PROPERTIES OF INVITRO PURKINJE-CELL DENDRITES IN MAMMALIAN CEREBELLAR SLICES
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DOI：
10.1113/jphysiol.1980.sp013358
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发表时间：
1980-01-01
影响因子：
5.5
通讯作者：
SUGIMORI, M
中科院分区：
文献类型：
--
作者：
LLINAS, R;SUGIMORI, M
体外豚鼠浦肯野细胞的树突内记录表明，白质刺激通过激活攀爬传入神经而产生了较大的突触反应，但反向电位没有主动侵入树突树。攀爬纤维的反应可能以类似于躯体水平的方式逆转。这种逆转在体细胞水平上没有双相性。这些树突的输入电阻范围为15-30 m ω…我们遇到了去极化电流在体细胞水平上的非线性特性。不太反常的整流是明显的。研究了直流外电流诱导浦肯野细胞的重复放电。在反体侵入时，快速体细胞电位（s.s）不主动侵入树突。树突突爆发（ds.b.s）在树突水平上最为突出。观察到两种类型的电压依赖性Ca响应。在低刺激水平下，电极呈高原样去极化，电导变化明显；进一步去极化产生大的树突动作电位。这两类反应是TTX[河蟹毒素]抗性的，但被Cd， Co， Mn或D600[甲氧基维拉帕米]或去除细胞外Ca阻断。在Ca电导阻断后，主要在体细胞附近观察到非失活的Na电导产生的平台电位。这种电压依赖性电导可能与体膜有关。浦肯野细胞树突的自发放电与在胞体中观察到的非常相似。这些脉冲的振幅在树突水平上更大。这些tttx不敏感的尖峰显然是在树突树的多个位置产生的。六种离子电导似乎与浦肯野细胞电反应有关：胞体或胞体附近的失活和非失活的Na电导，产生尖峰和高原的Ca电导，电压依赖和Ca依赖的K电流。讨论了这些电导在浦肯野细胞整合中的可能作用。
Intradendritic recordings from guinea pig Purkinje cells in vitro indicated that white matter stimulation produced large synaptic responses by the activation of the climbing afferent, but antidromic potentials did not actively invade the dendritic tree. Climbing fiber responses may be reversed in a manner similar to that at the somatic level. The reversal showed no biphasicity often seen at somatic level. Input resistance of these dendrites ranged from 15-30 M.OMEGA.. Non-linear properties seen at the somatic level for depolarizing currents were encountered. Less anomalous rectification was apparent. Repetitive firing of Purkinje cells elicited by outward DC current was analyzed. In antidromic invasion, the fast somatic potentials (s.s.) did not invade the dendrite actively. The dendritic spike bursts (d.s.b.s) interposed between the s.s. potentials were most prominent at dendritic level. Two types of voltage-dependent Ca responses were observed. At low stimulus level a plateau-like depolarization was accompanied by a prominent conductance change; further depolarization produced large dendritic action potentials. These 2 classes of response were TTX[tetrodo toxin]-resistant but were blocked by Cd, Co, Mn or D600 [methoxy verapamil] or the removal of extracellular Ca. Following blockage of the Ca conductance, plateau potentials produced by a non-inactivating Na conductance were observed mainly near the soma. This voltage-dependent conductance was probably associated with the somatic membrane. Spontaneous fiing in Purkinje cell dendrites was very similar to that observed at the soma. The amplitude of these bursts was larger at the dendritic level. These TTTX-insensitive spikes apparently were generated at multiple sites along the dendritic tree. Six ionic conductances seem involved in Purkinje cell electroresponsiveness: an inactivating and a non-inactivating Na conductance at or near the soma, a spike- and a plateau-generating Ca conductance, and voltage-dependent and Ca-dependent K currents. The possible role of these conductances in Purkinje cell integration is discussed.