Two-photon imaging of cortical surface microvessels reveals a robust redistribution in blood flow after vascular occlusion.
Two-photon imaging of cortical surface microvessels reveals a robust redistribution in blood flow after vascular occlusion.
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DOI：
10.1371/journal.pbio.0040022
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发表时间：
2006-02
期刊：
影响因子：
9.8
通讯作者：
Kleinfeld D
中科院分区：
文献类型：
--
作者：
Schaffer CB;Friedman B;Nishimura N;Schroeder LF;Tsai PS;Ebner FF;Lyden PD;Kleinfeld D
哺乳动物皮质上覆盖着一个高度相互连接的小动脉网络，将血液输送到皮质外套膜。在这里，我们测试这种血管结构是否可以确保血管闭塞后血液供应的重新分配。我们以啮齿动物顶叶皮质为模型系统，对单个微血管中红细胞的流动情况进行了成像。血流的变化是根据对单个软脑膜小动脉的光血栓闭塞以及大脑中动脉(MCA)的物理闭塞而进行量化的，大脑中动脉(MCA)是这个网络的主要血液来源。我们观察到，通过一个血管内血流的逆转，在光凝血栓闭塞下游的第一支血管上迅速重建血流灌注。更远端的下游小动脉也表现为血流倒置。此外，闭塞大脑中动脉导致大约一半的下游但远端小动脉的血流逆转。因此，皮质小动脉网络支持侧支循环，这可能减轻血管阻塞的影响，这可能是继发于神经血管病理的。作者量化了靶向阻断个别血管后啮齿动物皮质软脑膜小动脉网络中血流的变化。
A highly interconnected network of arterioles overlies mammalian cortex to route blood to the cortical mantle. Here we test if this angioarchitecture can ensure that the supply of blood is redistributed after vascular occlusion. We use rodent parietal cortex as a model system and image the flow of red blood cells in individual microvessels. Changes in flow are quantified in response to photothrombotic occlusions to individual pial arterioles as well as to physical occlusions of the middle cerebral artery (MCA), the primary source of blood to this network. We observe that perfusion is rapidly reestablished at the first branch downstream from a photothrombotic occlusion through a reversal in flow in one vessel. More distal downstream arterioles also show reversals in flow. Further, occlusion of the MCA leads to reversals in flow through approximately half of the downstream but distant arterioles. Thus the cortical arteriolar network supports collateral flow that may mitigate the effects of vessel obstruction, as may occur secondary to neurovascular pathology. The authors quantify changes in blood flow in the pial arteriolar network of rodent cortex following targeted occlusions to individual vessels.