High-performance liquid chromatographic purification of peptide hormones: ovine hypothalamic amunine (corticotropin releasing factor).
High-performance liquid chromatographic purification of peptide hormones: ovine hypothalamic amunine (corticotropin releasing factor).
复制标题
高效液相色谱纯化肽类激素：羊下丘脑胺（促肾上腺皮质激素释放因子）。
DOI：
10.1016/0003-2697(82)90170-1
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发表时间：
1982
影响因子：
2.9
通讯作者：
Vale,W
中科院分区：
文献类型：
--
作者：
Rivier,J;Rivier,C;Spiess,J;Vale,W
胺，或促肾上腺皮质激素释放因子（CRF），是一种41肽酰胺，已从绵羊下丘脑中分离出来。在通过凝胶渗透法测定大小之前，对组织进行浸提、脱脂和分配。活性区，然后通过一个敏感的体外试验（CRF介导的促肾上腺皮质激素释放的垂体细胞在单层培养物定量的特定的促肾上腺皮质激素放射免疫测定）可以纯化的反相高效液相色谱法。柱特征（即，讨论了衍生二氧化硅的性质，包括孔径）、移动的相的选择和温度效应。
Amunine, or corticotropin releasing factor (CRF), a 41-peptide amide, has been isolated from ovine hypothalami. Tissues were extracted, defatted, and partitioned prior to being sized by gel permeation. The active zone followed by a sensitive in vitro assay (CRF-mediated corticotropin release from pituitary cells in monolayer cultures quantitated by a specific corticotropin radioim munoassay) could be purified by reverse-phase high-performance liquid chromatography. Column characteristics (i.e., properties of the derivatized silicas including pore size), selection of mobile phases, and temperature effects are discussed.