Transplantation of human breast epithelia to mammary-gland-free fat-pads of athymic nude mice: influence of mammotrophic hormones on growth of breast epithelia.
Transplantation of human breast epithelia to mammary-gland-free fat-pads of athymic nude mice: influence of mammotrophic hormones on growth of breast epithelia.
复制标题
将人乳腺上皮移植到无胸腺裸鼠的无乳腺脂肪垫：促乳腺激素对乳腺上皮生长的影响。
DOI：
10.1002/ijc.2910410513
复制
发表时间：
1988
影响因子：
6.4
通讯作者：
Welsch,CW
中科院分区：
文献类型：
--
作者：
Sheffield,LG;Welsch,CW
Normal human breast tissue was enzymatically dissociated and the cells were injected Into the gland‐free fat‐pads of athymic nude mice. Within 30 days, small, spherical, duct‐like epithelial elements (organoids) formed in 68% of the fat‐pads inoculated (0‐23 organoids/fat‐pad). Short‐term (30‐day) treatment of the host mice with mammotrophic hormones [secretions from a choriunic, somato‐mammotrophin‐secreting transplantable human choriocarcinoma (JEG‐3), secretions from a prolactin‐ and growth‐hormone‐secreting transplant‐able rat pituitary tumor (GH3), estrogen and/or progesterone] and/or cAMP inducers (cholera toxin) significantly (p<0.05) increased the size of the human breast organoids but did not increase organoid number or induce extensive and expansive growth (extensive duct elongation and branching) of these structures. Such treatments induced intense proliferation of the host mouse mammae resembling that which occurs during late pregnancy. The results of this study, therefore, provide evidence that normal human breast epithelium can be readily accepted by and maintained in the gland‐free fat‐pad of the athymic nude mouse, and the epithelium, within 10 days, forms spherical duct‐like structures (organoids). The human breast organoids are hormone‐responsive, as they respond to a mammotrophic growth stimulus by an increase in size. The failure of the human breast organoids to grow expansively in the gland‐free fat‐pad of this immunologically deficient mouse does not appear to be due to the absence of an appropriate hormonal growth stimulus.
