Gene Signature of Human Oral Mucosa Fibroblasts: Comparison with Dermal Fibroblasts and Induced Pluripotent Stem Cells.
Gene Signature of Human Oral Mucosa Fibroblasts: Comparison with Dermal Fibroblasts and Induced Pluripotent Stem Cells.
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DOI：
10.1155/2015/121575
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发表时间：
2015
影响因子：
--
通讯作者：
Noma T
中科院分区：
文献类型：
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作者：
Miyoshi K;Horiguchi T;Tanimura A;Hagita H;Noma T
口腔黏膜是一种有用的再生治疗材料，其优点是可及性和多功能性，无论年龄和性别。然而，对口腔黏膜的分子特性了解甚少。在这里，我们报告了人类口腔黏膜成纤维细胞（hOFs）、人类真皮成纤维细胞（hDFs）和hof衍生的诱导多能干细胞（hOF-iPSCs）的基因特征的首次比较，并通过功能注释和途径分析将这些基因特征与生物学作用联系起来。作为成纤维细胞的共同特征，与hOF-iPSCs相比，hof和hDFs都表达了更高水平的糖脂代谢相关基因。与hDFs相比，hfs的显著特征包括高表达糖蛋白基因（涉及信号、细胞外基质、膜和受体蛋白），以及低表达HOX基因（hDFs的标志物）。通路分析结果表明，组织重建、增殖和信号通路是活跃的，而p53通路中的衰老相关基因在hof中是不活跃的。此外，超过一半的hof特异性基因与hOF-iPSC基因表达相似，可能受WNT信号控制。我们的研究结果表明，hof在特异性和可塑性方面具有独特的细胞特征。这些数据可能为口腔成纤维细胞直接重编程的应用提供有用的见解。
Oral mucosa is a useful material for regeneration therapy with the advantages of its accessibility and versatility regardless of age and gender. However, little is known about the molecular characteristics of oral mucosa. Here we report the first comparative profiles of the gene signatures of human oral mucosa fibroblasts (hOFs), human dermal fibroblasts (hDFs), and hOF-derived induced pluripotent stem cells (hOF-iPSCs), linking these with biological roles by functional annotation and pathway analyses. As a common feature of fibroblasts, both hOFs and hDFs expressed glycolipid metabolism-related genes at higher levels compared with hOF-iPSCs. Distinct characteristics of hOFs compared with hDFs included a high expression of glycoprotein genes, involved in signaling, extracellular matrix, membrane, and receptor proteins, besides a low expression of HOX genes, the hDFs-markers. The results of the pathway analyses indicated that tissue-reconstructive, proliferative, and signaling pathways are active, whereas senescence-related genes in p53 pathway are inactive in hOFs. Furthermore, more than half of hOF-specific genes were similarly expressed to those of hOF-iPSC genes and might be controlled by WNT signaling. Our findings demonstrated that hOFs have unique cellular characteristics in specificity and plasticity. These data may provide useful insight into application of oral fibroblasts for direct reprograming.