Laboratory transmission of La Crosse virus by Ochlerotatus j. japonicus (Diptera: Culicidae)
Laboratory transmission of La Crosse virus by Ochlerotatus j. japonicus (Diptera: Culicidae)
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DOI：
10.1603/0022-2585-39.4.635
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发表时间：
2002-07-01
影响因子：
2.1
通讯作者：
Andre, ARG
中科院分区：
文献类型：
--
作者：
Sardelis, MR;Turell, MJ;Andre, ARG
研究了最近引入美国的黄足金龟，以确定其作为拉克罗斯（LAC）病毒载体的能力。对现场收集的三列黄腹小蜂（LAC病毒的主要载体）种群进行了类似的测试以进行比较。在Oc. j.从仓鼠中摄取病毒，病毒密度为10（3.6-5.4）个噬斑形成单位（PFU）/ ml血液，其估计传播率为35- 88%。这些比率略低于OC，但与OC相似。三列虫75- 100%。Oc中的病毒滴度。j.在摄入含LAC病毒浓度为10（5.4）PFU/ml血液的血粉后约7 d，病毒浓度达到峰值，约为10（5.5）PFU/只蚊子; 100%（8/8）的标本中病毒已从中肠传播。这些数据，结合OC的栖息地之间的密切联系。j. J.和Oc. triseriatus和报道的范围扩大，这一新发现的物种在美国东部，表明Oc。j. japonicus可以作为LAC病毒的额外载体。
Ochlerotatus j. japonicus, a recent introduction to the United States, was studied to determine its capability to serve as a vector of La Crosse (LAC) virus. A field-collected population of Ochlerotatus triseriatus, the primary vector of LAC virus, was similarly tested for comparison. After Oc. j. japonicus ingested virus from hamsters with viremias of 10(3.6-5.4) plaque-forming units (PFU) / ml of blood, its estimated transmission rates were 35-88%. These rates were slightly lower than, though similar to, those for Oc. triseriatus, 75-100%. Viral titers in Oc. j. japonicus peaked at approximate to10(5.5) PFU/ mosquito about 7 d after ingesting a blood meal in which the concentration of LAC virus was 10(5.4) PFU/ml of blood; virus had disseminated from the midgut in 100% (8/8) of these specimens. These data, combined with the close association between the habitats of Oc. j. japonicus and Oc. triseriatus and the reported expansion of the range of this newly discovered species in the eastern United States, indicate that Oc. j. japonicus could function as an additional vector of LAC virus.