Serum surfactant protein D is increased in acute and chronic inflammation in mice
Serum surfactant protein D is increased in acute and chronic inflammation in mice
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DOI：
10.1016/j.cyto.2005.02.006
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发表时间：
2005-07-07
期刊：
影响因子：
3.8
通讯作者：
Mason, RJ
中科院分区：
文献类型：
--
作者：
Fujita, M;Shannon, JM;Mason, RJ
表面活性蛋白 A (SP-A) 和表面活性蛋白 D (SP-D) 是先天免疫的重要组成部分，可以改变炎症反应。然而，急性和慢性炎症中 SP-A 和 SP-D 的改变和调节尚不清楚。此外，血清SP-D可能作为肺部炎症的生物标志物。我们测定了小鼠模型中SP-A和SP-D的表达。为了研究急性炎症，我们在支气管内滴注博莱霉素。为了研究慢性肺部炎症，我们使用了过度表达肿瘤坏死因子 (TNF)-α 的转基因小鼠。在 SP-C 启动子的控制下。这些小鼠具有慢性单核细胞浸润、空腔扩大、肺动脉高压和局灶性肺纤维化。在急性炎症模型中，给予博莱霉素后所有表面活性蛋白的mRNA水平均降低。然而，滴注后第 7 天至第 28 天血清 SP-D 有所增加。在慢性炎症模型中，SP-D mRNA表达增加，而SP-A、SP-B和SP-C表达减少。在慢性肺部炎症中，血清和肺部 SP-D 浓度均升高。这些数据阐明了急性和慢性炎症中 SP-A 和 SP-D 的分布，表明血清 SP-D 可以作为小鼠急性和慢性肺损伤中肺部炎症的生物标志物。 (c) 2005 Elsevier Ltd. 保留所有权利。
Surfactant protein A (SP-A) and surfactant protein D (SP-D) are important components of innate immunity that can modify the inflammatory response. However, alterations and regulation of SP-A and SP-D in acute and chronic inflammation are not well defined. In addition, serum SP-D may serve as a biomarker of lung inflammation.We determined the expression of SP-A and SP-D in murine models. To study acute inflammation, we instilled bleomycin intrabronchially. To study chronic lung inflammation, we used a transgenic mouse that overexpresses tumor necrosis factor (TNF)-alpha. under the control of the SP-C promoter. These mice have a chronic mononuclear cell infiltration, airspace enlargement, pulmonary hypertension, and focal pulmonary fibrosis.In acute inflammation model, levels of mRNA for all surfactant proteins were reduced after bleomycin administration. However, serum SP-D was increased from days 7 to 28 after instillation. In chronic inflammation model, SP-D mRNA expression was increased, whereas the expression of SP-A, SP-B and SP-C was reduced. Both serum and lung SP-D concentrations were increased in chronic lung inflammation.These data clarified profile of SP-A and SP-D in acute and chronic inflammation and indicated that serum SP-D can serve as a biomarker of lung inflammation in both acute and chronic lung injury in mice. (c) 2005 Elsevier Ltd. All rights reserved.