Thio-glucose bound gold nanoparticles enhance radio-cytotoxic targeting of ovarian cancer
Thio-glucose bound gold nanoparticles enhance radio-cytotoxic targeting of ovarian cancer
复制标题
硫代葡萄糖结合的金纳米颗粒增强卵巢癌的放射细胞毒性靶向
DOI：
10.1088/0957-4484/22/28/285101
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发表时间：
2011-07-15
期刊：
影响因子：
3.5
通讯作者：
Kong, Beihua
中科院分区：
文献类型：
--
作者：
Geng, Feng;Song, Kun;Kong, Beihua
卵巢癌的治疗传统上是棘手的，需要新的方法来提高治疗效率。本文报道了硫代葡萄糖结合的金纳米颗粒（Glu-GNP）可作为增敏剂用于增强卵巢癌的放射治疗。人卵巢癌细胞SK-0 V-3通过单独的金纳米颗粒（GNP）、单独的照射或GNP加照射处理。使用电感耦合等离子体原子发射光谱法（ICP-AES）测定细胞摄取，同时使用3-（4，5）-二甲基噻唑（-z-y1）-3，5-二苯基四唑溴化物和克隆形成试验测定放射治疗诱导的细胞毒性。使用CM-H2-DCFDA共聚焦显微镜测定活性氧（ROS）的存在，并通过Annexin V-FITC/碘化丙啶（PI）试剂盒和流式细胞术测定细胞凋亡。与裸GNP相比，用Glu-GNP处理的细胞导致纳米颗粒摄取增加约31%（p < 0.005）。与单独照射处理相比，细胞内摄取Glu-GNP导致90 kVp和6 MV照射对细胞增殖的抑制分别增加30.48%和26.88%。X射线辐射与GNP的相互作用诱导ROS产生水平升高，这是GNP可以增强卵巢癌放射治疗的机制之一。
The treatment of ovarian cancer has traditionally been intractable, and required novel approaches to improve therapeutic efficiency. This paper reports that thio-glucose bound gold nanoparticles (Glu-GNPs) can be used as a sensitizer to enhance ovarian cancer radiotherapy. The human ovarian cancer cells, SK-OV-3, were treated by gold nanoparticles (GNPs) alone, irradiation alone, or GNPs in addition to irradiation. Cell uptake was assayed using inductively coupled plasma atomic emission spectroscopy (ICP-AES), while cytotoxicity induced by radiotherapy was measured using both 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide and clonogenic assays. The presence of reactive oxygen species (ROS) was determined using CM-H2-DCFDA confocal microscopy and cell apoptosis was determined by an Annexin V-FITC/propidium iodide (PI) kit with flow cytometry. The cells treated by Glu-GNPs resulted in an approximate 31% increase in nanoparticle uptake compared to naked GNPs (p < 0.005). Compared to the irradiation alone treatment, the intracellular uptake of Glu-GNPs resulted in increased inhibition of cell proliferation by 30.48% for 90 kVp and 26.88% for 6 MV irradiation. The interaction of x-ray radiation with GNPs induced elevated levels of ROS production, which is one of the mechanisms by which GNPs can enhance radiotherapy on ovarian cancer.