The biological characters of Bmelav-like genes in the development of Bombyx mori.
The biological characters of Bmelav-like genes in the development of Bombyx mori.
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家蚕发育过程中Bmelav样基因的生物学特性。
DOI：
10.1111/imb.12668
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发表时间：
--
影响因子：
2.6
通讯作者：
Li M
中科院分区：
文献类型：
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作者：
Sun X;Zhang K;Gu J;Yang J;Huang Q;Yan R;Qin S;Hou C;Zhang G;Wang S;Li M
ELAV/Hu家族是一个保守的泛神经元RNA结合蛋白家族，参与后生动物转录后调控。在果蝇中，该家族的三个成员ELAV、RBP9和FNE参与神经元分化、基因表达调控等。在家蚕中，这个家庭的特征就不那么好了。BmELAV-like-1(Bmel-1)和BmELAV-like-2(Bmel-2)与果蝇和人类的同源性分别为55%-71%和47%-62%，Bmel-1广泛表达，Bmel-2在头部和卵巢特异表达。这两个基因编码的蛋白质都定位在细胞核和细胞质中。利用CRISPR/CAS9技术创造的Bme1-1−/−2−突变体对蚕体、茧层重量的影响程度不同。这两个基因的突变增加了4个丝蛋白基因Fib-L、Fib-H、P25和Ser-1的表达。此外，Bmel-2−雌性的产卵能力下降。本研究不仅为Bmelav类基因在蚕丝生长发育、蚕茧性状和蚕丝蛋白基因表达调控中的功能研究提供了有价值的见解，也为家蚕品种选育提供了有用的信息。
The ELAV/Hu family is a conserved multigene family of pan‐neuronal RNA‐binding protein involved in post‐transcriptional regulation in metazoans. InDrosophila, three members of this family, ELAV, RBP9 and FNE, are involved in neuronal differentiation, gene expression regulation and so on. This family is less well characterized inBombyx mori. Two orthologs BmELAV‐like‐1 (BmEL‐1) and BmELAV‐like‐2 (BmEL‐2) share 55%–71% and 47%–62% identity with that of inDrosophilaand humans, respectively.Bmel‐1is ubiquitously expressed whileBmel‐2is expressed in the head and ovaries specifically. Proteins encoded by both genes are localized in nuclear and cytoplasm. The weight of body, cocoon, pupae and cocoon shell are differently affected inBmel‐1−/−2−mutants created using CRISPR/Cas9 technology. Mutations of both genes increase the expression of four silk protein genes,Fib‐L,Fib‐H,P25andSer‐1. In addition, the oviposition ability ofBmel‐2−females is decreased. This study not only provides valuable insights into the functional roles ofBmelav‐likegenes in the growth, cocoon characters and regulation of silk protein genes expression, but also provides useful information for silkworm variety breeding.