An α-glucan isolated from root of Isatis Indigotica, its structure and adjuvant activity
An α-glucan isolated from root of Isatis Indigotica, its structure and adjuvant activity
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DOI：
10.1007/s10719-014-9525-y
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发表时间：
2014-05-01
影响因子：
3
通讯作者：
Wang, Yuxia
中科院分区：
文献类型：
--
作者：
Shan, Junjie;Sun, Guohui;Wang, Yuxia
The root of Isatis indigotica is a traditional Chinese herbal medicine. An alpha-glucan (IIP-A-1) was firstly isolated from the roots. In this study we elucidated the chemical structure of IIP-A-1 and determined its adjuvant activity by co-immunizing mice with H1N1 influenza virus split and recombinant hepatitis B surface antigen (HBsAg), respectively. The polysaccharide was pretreated with periodate oxidation, Smith degradation and methylation in order to analyze its structure using GC, HPGPC, FT-IR, NMR and GC-MS. The adjuvant effect was evaluated by determining the antibody titers of serum against H1N1 influenza and HBsAg using ELISA. The proliferation and TNF-alpha secretion of macrophages administrated with different dose of IIP-A-1 were measured in vitro. The results of this study revealed that IIP-A-1 was an alpha-glucan with the molecular weight of 3,600 Da. The backbone was alpha-(1 -> aEuro parts per thousand 4)-D-glucan with (1 -> aEuro parts per thousand 6) branch chain. The alpha-glucan could significantly enhance the immune response of mice immunized with H1N1 influenza or HBsAg in vivo and exert good dose-dependent effects on the proliferation and the TNF-alpha secretion of macrophages in vitro. These results supported that IIP-A-1 was expected to be an efficacious adjuvant candidate for prophylactic and therapeutic vaccines.
