Antibodies specific for the polyoma virus middle-size tumor antigen.
Antibodies specific for the polyoma virus middle-size tumor antigen.
复制标题
多瘤病毒中型肿瘤抗原特异性抗体。
DOI：
10.1073/pnas.78.8.4882
复制
发表时间：
1981
影响因子：
11.1
通讯作者：
Eckhart,W
中科院分区：
文献类型：
--
作者：
Walter,G;Hutchinson,MA;Hunter,T;Eckhart,W
我们用合成肽Lys-Arg-Ser-Arg-His-Phe免疫兔，获得了针对多瘤病毒中型肿瘤抗原(中T抗原)的抗体。抗肽血清沉淀多瘤中T抗原，而不沉淀小或大的肿瘤抗原，并且在多肽的存在下，沉淀被抑制。两种细胞蛋白，30,000和26,000道尔顿，也被抗肽血清特异性地沉淀，并可能具有与该肽相关的氨基酸序列。另外两种细胞蛋白，33,000和25,000道尔顿，只有在多肽存在的情况下才会沉淀，并可能在细胞提取物中与之相关。针对合成肽的抗血清可能在各种方面都很重要，包括产生针对特定决定簇的抗体，以及识别其基因已被分析的未知蛋白质。
We have obtained antibodies specific for the polyoma virus middle-size tumor antigen (middle T antigen) by immunizing rabbits with a synthetic peptide, Lys-Arg-Ser-Arg-His-Phe, corresponding to the six carboxy-terminal amino acids of the middle T antigen predicted from the nucleotide sequence of polyoma DNA. The antipeptide serum precipitates the polyoma middle T antigen but not the small or large tumor antigens, and precipitation is inhibited in the presence of the peptide. Two cellular proteins, 30,000 and 26,000 daltons, are also precipitated specifically by the antipeptide serum and may have amino acid sequences related to the peptide. Two other cellular proteins, 33,000 and 25,000 daltons, are precipitated only in the presence of the peptide and may associate with it in cell extracts. Antisera directed against synthetic peptides are likely to be important in various ways, including the production of antibodies directed against particular determinants and the recognition of unknown proteins whose genes have been analyzed.