Analysis of adenosine triphosphate and glutathione through gold nanoparticles assisted laser desorption/ionization mass spectrometry
Analysis of adenosine triphosphate and glutathione through gold nanoparticles assisted laser desorption/ionization mass spectrometry
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DOI：
10.1021/ac070023x
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发表时间：
2007-07-01
影响因子：
7.4
通讯作者：
Chang, Huan-Tsung
中科院分区：
文献类型：
--
作者：
Huang, Yu-Fen;Chang, Huan-Tsung
本文介绍了使用适配体修饰的金纳米粒子（Apt-AuNPs）作为选择性探针和AuNPs作为表面辅助激光解吸/电离（SALDI）矩阵的三磷酸腺苷（ATP）的质谱（MS）测定。将适体共价连接到AuNP的表面以形成提供对ATP的选择性的Apt-AuNP。然而，当与AuNP相比时，Apt-AuNP是效率较低的激光解吸/电离（LDI）基质。通过使用Apt-AuNPs作为选择性探针和AuNPs作为LDI基质，MS方法提供了ATP的检测限（LOD），信噪比为0.48 μ M的3。当与常规有机基质（例如，由于AuNPs具有良好的抗氧化性（例如2，5-二羟基苯甲酸），因此AuNPs作为LDI基质提供了许多优点，包括易于制备、选择性、灵敏度和可重复性。已经证明了分别使用Apt-AuNP和AuNP通过SALDI以阴性和阳性MS模式对人细胞裂解物中的ATP和GSH进行顺序分析。本研究结果表明，通过测定ATP和GSH的浓度来监测细胞的生物活性的方法的实用性。
This paper describes the use of aptamer-modified gold nanoparticles (Apt-AuNPs) as selective probes and AuNPs as the surface-assisted laser desorption/ionization (SALDI) matrixes for the determination of adenosine triphosphate (ATP) by mass spectrometry (MS). The aptamers were covalently attached to the surface of AuNPs to form Apt-AuNPs that provided selectivity toward ATP. However, Apt-AuNPs are less efficient laser desorption/ionization (LDI) matrixes when compared to AuNPs. By using Apt-AuNPs as selective probes and AuNPs as LDI matrixes, the MS approach provided the limit of detection (LOD) for ATP at a signal-to-noise ratio of 3 of 0.48 mu M. When compared to conventional organic matrixes (e.g., 2,5-dihydroxybenzoic acid), AuNPs as LDI matrixes provide a number of advantages, including ease of preparation, selectivity, sensitivity, and repeatability. Sequential analysis of ATP and GSH in human cell lysates by SALDI with negative and positive MS modes, respectively, using Apt-AuNPs and AuNPs has been demonstrated. The present results demonstrate the practicality of the approach for monitoring the bioactivity of cells through determinations of the concentrations of ATP and GSH.