A glycoprotein M-deleted equid herpesvirus 4 is severely impaired in virus egress and cell-to-cell spread.
A glycoprotein M-deleted equid herpesvirus 4 is severely impaired in virus egress and cell-to-cell spread.
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糖蛋白 M 缺失的马科动物疱疹病毒 4 的病毒输出和细胞间传播严重受损。
DOI：
10.1099/vir.0.80393-0
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
A. Neubauer
中科院分区：
文献类型：
--
作者：
Christina Ziegler;F. Just;A. Lischewski;K. Elbers;A. Neubauer
为了分析马疱疹病毒4型（EHV-4）糖蛋白M同源物（gM）的功能，产生了两种不同的突变病毒（E4 DeltagM-GFP和E4 DeltagM-w）。两种GM阴性EHV-4突变体在互补和非互补细胞上进行表征，并与E4 RgM（一种已修复GM表达的病毒）进行比较。在病毒生长动力学中证明，删除gM对EHV-4复制的影响比预期的更显著。比EHV-4感染的Vero细胞晚9-12 h检测到细胞外感染性，滴度降低至2000倍。此外，斑块的平均最大直径小于野生型斑块直径的20%。这些结果与大多数其他α疱疹病毒相反，包括密切相关的马疱疹病毒1型，其中gM的缺失仅轻微影响病毒在细胞培养物中复制的能力。然而，通过电子显微镜分析感染的细胞没有发现删除gM的特定缺陷。得出的结论是，EHV-4 gM是重要的病毒复制在细胞培养中的一个以上的步骤，影响有效的病毒出口和细胞间的传播。
To analyse the function of the equid herpesvirus 4 (EHV-4) glycoprotein M homologue (gM), two different mutated viruses (E4DeltagM-GFP and E4DeltagM-w) were generated. Both gM-negative EHV-4-mutants were characterized on complementing and on non-complementing cells and compared with E4RgM, a virus where gM-expression had been repaired. It was demonstrated in virus growth kinetics that deleting gM had a more dramatic influence on EHV-4 replication than expected. Extracellular infectivity was detected 9-12 h later than in EHV-4-infected Vero cells and titres were reduced up to 2000-fold. In addition, mean maximal diameters of plaques were less than 20 % of diameters of wild-type plaques. These results are in contrast to most other alphaherpesviruses, including the closely related equid herpesvirus type 1, where deletion of gM only marginally influences the ability of viruses to replicate in cell culture. Nevertheless, analysis of infected cells by electron microscopy did not reveal a specific defect for deleting gM. It was concluded that EHV-4 gM is important for more than one step in virus replication in cell culture, influencing both efficient virus egress and cell-to-cell spread.
马疱疹病毒1型糖蛋白14的合成和加工。
影响因子：
3.7
作者：
Sullivan,DC;Allen,GP;O'Callaghan,DJ
通讯作者：
O'Callaghan,DJ