Antigenic relatedness of stocks and clones of Trypanosoma vivax from East and West Africa
Antigenic relatedness of stocks and clones of Trypanosoma vivax from East and West Africa
复制标题
东非和西非间日锥虫种群和克隆的抗原相关性
作者：
G. J. Vos;P. Gardiner
采用免疫裂解法比较了东非和西非7个间日锥虫种群和7个间日锥虫克隆的抗原关系。用不同株系和克隆的T.在感染后第40天和第80天收集的间日疟原虫用于与同源和异源的锥虫原种和克隆的免疫裂解试验。包括来自感染牛的血清以比较来自肯尼亚的种群和克隆。在免疫溶解试验中用作抗原的寄生虫，当使用可变抗原类型（VAT）同质种群时，从感染小鼠中收集，从山羊中收集，用于感染来自尼日利亚、冈比亚和乌干达的种群和克隆，从牛中收集，用于肯尼亚种群。在来自尼日利亚和冈比亚的所有储备和克隆之间发现了血清和寄生虫之间的相互交叉反应性，但来自尼日利亚的一个克隆除外，该克隆不被来自冈比亚的克隆的抗血清识别。来自乌干达的原种和克隆与来自尼日利亚和冈比亚的原种和克隆之间也存在交叉反应性。来自感染来自尼日利亚、冈比亚和乌干达的种群和克隆的山羊的血清识别寄生虫种群，其对于来自尼日利亚的啮齿动物感染性种群的一种VAT（ILDat 1.2）是同质的。一些抗血清西非股票承认另一个稳定的变种，从乌干达股票适应啮齿动物（ILDat 2.1），表明这些增值税的表达在库的异源股票。来自尼日利亚、冈比亚或乌干达的种群与肯尼亚种群之间没有交叉反应。来自Galana（肯尼亚）和Bamburi（肯尼亚）的股票显示出相互交叉反应性。来自Kilifi和Likoni的另外两个肯尼亚种群也通过免疫裂解显示出交叉反应性，但与其他肯尼亚种群没有抗原关系。
Summary The antigenic relationships of 7 stocks and 7 clones of Trypanosoma vivax from East and West Africa were compared by immune lysis. Sera from goats infected with different stocks and clones of T. vivax, collected on days 40 and 80 after infection, were used in the immune lysis test with homologous and heterologous stocks and clones of trypanosomes. Sera from infected cattle were included to compare stocks and clones from Kenya. The parasites that were used as antigen in the immune lysis tests were collected from infected mice when variable antigen type (VAT) homogeneous populations were used, from goats for infection with stocks and clones from Nigeria, The Gambia and Uganda, and from cattle for Kenyan stocks. Reciprocal cross-reactivity between sera and parasites was found between all the stocks and clones from Nigeria and The Gambia with the exception of one clone from Nigeria that was not recognized by antisera to a clone from The Gambia. There was also cross-reactivity between a stock and clone from Uganda and stocks and clones from Nigeria and The Gambia. Sera from goats infected with stocks and clones from Nigeria, The Gambia and Uganda recognized parasite populations that were homogeneous for one VAT (ILDat 1.2) of the rodent infective stock from Nigeria. Some antisera to West African stocks recognized another stable variant from a Ugandan stock adapted to rodents (ILDat 2.1), indicating that these VATs were expressed in the repertoires of the heterologous stocks. There was no cross-reaction between stocks from Nigeria, The Gambia or Uganda with Kenyan stocks. A stock from Galana (Kenya) and Bamburi (Kenya) showed reciprocal cross-reactivity. Two other Kenyan stocks, from Kilifi and Likoni, also showed cross-reactivity by immune lysis but showed no antigenic relationship with the other Kenyan stocks.