MICROBIAL DETERMINATIONS BY FLOW CYTOMETRY
MICROBIAL DETERMINATIONS BY FLOW CYTOMETRY
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DOI：
10.1099/00221287-113-2-369
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
EIPEL, HE
中科院分区：
文献类型：
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作者：
HUTTER, KJ;EIPEL, HE
流式细胞术系统的光学和电子学以及染色技术的最新改进允许对微生物的DNA和蛋白质含量等微小细胞成分进行测定。为了评估这种技术的有用性，在大肠杆菌，短乳杆菌，干酪乳杆菌，小球藻kessleri 8k，酿酒酵母，产朊假丝酵母，粟酒裂殖酵母和Euglena gracilis中测定DNA和蛋白质含量分布。对多倍体酿酒酵母DNA含量分布的研究表明，该方法可用于倍性鉴定。流式细胞术测量的快速性允许在大群体中进行准确测定。
Recent improvements in the optics and electronics of flow cytometry systems and in staining techniques permit the assay of such minute cellular constituents as the DNA and protein contents of microorganisms. To assess the usefulness of this technique, DNA and protein content distributions were determined in Escherichia coli, Lactobacillus brevis, Lactobacillus casei, Chlorella kessleri 8k, Saccharomyces cerevisiae, Candida utilis, Schizosaccharomyces pombe and Euglena gracilis. Investigations of the DNA content distributions of polyploid strains of Saccharomyces cerevisiae indicated that the method can be used to determine ploidy. The rapidity of flow cytometry measurements allows accurate determinations in large populations.