Molecular epidemiological and virological study of dengue virus infections in Guangzhou, China, during 2001-2010.
Molecular epidemiological and virological study of dengue virus infections in Guangzhou, China, during 2001-2010.
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DOI：
10.1186/1743-422x-10-4
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发表时间：
2013-01-02
期刊：
影响因子：
4.8
通讯作者：
Wang M
中科院分区：
文献类型：
--
作者：
Jiang L;Wu X;Wu Y;Bai Z;Jing Q;Luo L;Dong Z;Yang Z;Xu Y;Cao Y;Di B;Wang Y;Wang M
登革病毒（DENV）感染是全球热带和亚热带地区最流行的节肢动物传播病毒感染。广州是登革病毒传播的理想环境，登革病毒在该地区的流行已有30多年的报道。方法收集广州市2001 - 2010年登革病毒感染病例资料进行分析。从患者血清中培养分离登革病毒株。从细胞培养上清液中提取病毒RNA。通过逆转录PCR合成cDNA。分别构建了4个DENV血清型的系统发育树。总共报告了2478例DENV感染病例;其中2143例（86.43%）发生在一年中的3个月：8月，9月和10月。其中本地病例2398例（96.77%），外地输入病例80例（3.23%）。在输入性病例中，69例（86.25%）来自东南亚国家。90株分离株中，血清型1型占66.67%，血清型2型占3.33%，血清型3型占14.44%，血清型4型占15.56%。DENV-1在大多数年份中占主导地位，包括2002年和2006年的2次暴发;然而，本研究中发现的菌株或基因型均未占主导地位。有趣的是，来自不同年份的DENV毒株具有不同的起源。此外，每年的菌株属于不同的血清型和/或基因型。东南亚国家可能是广州市登革病毒的来源。这些结果表明，有越来越多的DENV菌株在广州，这可能会增加在不久的将来DENV爆发的风险。
Dengue virus (DENV) infection is the most prevalent arthropod-borne viral infection in tropical and subtropical regions worldwide. Guangzhou has the ideal environment for DENV transmission and DENV epidemics have been reported in this region for more than 30 years. Information for DENV infection cases in Guangzhou from 2001 to 2010 were collected and analyzed. The DENV strains were cultured and isolated from patients’ sera. Viral RNA was extracted from cell culture supernatants. cDNA was synthesized by reverse transcription PCR. Phylogenetic trees of four DENV serotypes were constructed respectively. In total, 2478 DENV infection cases were reported; 2143 of these (86.43%) occurred during 3 months of the year: August, September and October. Of these, 2398 were local cases (96.77%) and 80 were imported cases (3.23%). Among the imported cases, 69 (86.25%) were from Southeast Asian countries. From the 90 isolated strains, 66.67%, 3.33%, 14.44%, and 15.56% belonged to DENV serotypes 1, 2, 3, and 4, respectively. DENV-1 was predominant in most of the years, including during 2 outbreaks in 2002 and 2006; however, none of the strains or genotypes identified in this study were found to be predominant. Interestingly, DENV strains from different years had different origins. Moreover, the strains from each year belonged to different serotypes and/or genotypes. Southeast Asia countries were found to be the possible source of DENV in Guangzhou. These findings suggest that there is increasing diversity in DENV strains in Guangzhou, which could increase the risk of DENV outbreaks in the near future.