The use of fluorescence-activated cell sorting in studying plant development and environmental responses.
The use of fluorescence-activated cell sorting in studying plant development and environmental responses.
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DOI：
10.1387/ijdb.130195mg
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发表时间：
2013-09
期刊：
影响因子：
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通讯作者：
A. Carter;Roxanna Bonyadi;M. Gifford
中科院分区：
文献类型：
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作者：
A. Carter;Roxanna Bonyadi;M. Gifford
荧光激活细胞分选（FACS）是一种强大的工具，使植物的生长和发育，在细胞水平上进行研究。流式细胞术用于分离细胞亚群，例如特定细胞类型的细胞，或已用荧光蛋白标记的处于特定发育阶段的细胞。转基因技术使我们能够产生表达荧光蛋白的植物，不仅在特定细胞类型中组成型表达，而且还动态地响应内源性或外部因素。通过用FACS处理这样的转基因系，可以分离处于广泛的可能响应状态的不同细胞群体用于进一步分析。这对于研究植物中的生物学机制特别有用，因为生长和发育的控制在细胞类型水平上是明显的。此外，所得到的数据的特异性使得能够对转录网络进行精细建模，该转录网络对转录组进行系统级控制;因此可以识别植物中响应和过程的关键调控因子。在这篇综述中，通过细胞分选研究的案例研究，探讨了植物中流式细胞仪方法的当前状态，使我们能够做出重大的新发现。
Fluorescence-Activated Cell Sorting (FACS) is a powerful tool that enables plant growth and development to be studied at the cellular level. Flow cytometry is used to isolate subpopulations of cells, such as those of specific cell types, or cells at particular developmental stages that have been marked with fluorescent proteins. Transgenic technology has given us the ability to generate plants that express fluorescent proteins, not just constitutively in particular cell types, but also dynamically in response to endogenous or external factors. By processing such transgenic lines with FACS, it is possible to isolate distinct populations of cells in a wide range of likely response states for further analysis. This is particularly useful for investigating biological mechanisms in plants because the control of growth and development is manifest at the cell type level. Furthermore, the specificity of the resulting data enables fine modelling of the transcriptional networks that exert systems-level control of the transcriptome; hence key regulators of responses and processes in the plant can be identified. In this review, the current state of the art for FACS methods in plants is explored by means of case studies of research in which cell sorting allowed us to make significant new discoveries.