Microtubule-dependent transport of arenavirus matrix protein demonstrated using live-cell imaging microscopy.
Microtubule-dependent transport of arenavirus matrix protein demonstrated using live-cell imaging microscopy.
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使用活细胞成像显微镜证明了沙粒病毒基质蛋白的微管依赖性运输。
DOI：
10.1093/jmicro/dfz034
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Noda T.
中科院分区：
文献类型：
--
作者：
Takamatsu Y;Kajikawa J;Muramoto Y;Nakano M;Noda T.
拉沙病毒（LASV）属于沙粒病毒科，引起严重的出血表现，并与人类的高死亡率相关。因此，它被归类为生物安全水平（BSL）-4剂。由于LASV疾病的对策尚未开发，因此重要的是阐明病毒生命周期的分子机制，包括其病毒和宿主细胞蛋白质的相互作用。这些潜在的分子机制可能是开发新的治疗选择的关键。淋巴细胞性脉络丛脑膜炎病毒（LCMV）是LASV的近亲，通常无症状，被归类为BSL-2因子。在本研究中，我们可视化的运输病毒基质Z蛋白在LCMV感染的细胞使用活细胞成像显微镜。我们证明了Z蛋白的转运是由聚合的微管介导的。有趣的是，LASV Z蛋白在LCMV感染细胞中的转运表现出与Z蛋白相似的特征。使用LCMV的活细胞成像系统为在BSL-2实验室中研究沙粒病毒基质蛋白转运提供了有吸引力的替代措施。此外，它还可以用于分析病毒基质蛋白和细胞骨架之间的相互作用，以及评估靶向病毒基质蛋白转运的抗病毒化合物。
Lassa virus (LASV), belonging to the familyArenaviridae, causes severe haemorrhagic manifestations and is associated with a high mortality rate in humans. Thus, it is classified as a biosafety level (BSL)-4 agent. Since countermeasures for LASV diseases are yet to be developed, it is important to elucidate the molecular mechanisms underlying the life cycle of the virus, including its viral and host cellular protein interactions. These underlying molecular mechanisms may serve as the key for developing novel therapeutic options. Lymphocytic choriomeningitis virus (LCMV), a close relative of LASV, is usually asymptomatic and is categorized as a BSL-2 agent. In the present study, we visualized the transport of viral matrix Z protein in LCMV-infected cells using live-cell imaging microscopy. We demonstrated that the transport of Z protein is mediated by polymerized microtubules. Interestingly, the transport of LASV Z protein showed characteristics similar to those of Z protein in LCMV-infected cells. The live-cell imaging system using LCMV provides an attractive surrogate measure for studying arenavirus matrix protein transport in BSL-2 laboratories. In addition, it could be also utilized to analyze the interactions between viral matrix proteins and the cellular cytoskeleton, as well as to evaluate the antiviral compounds that target the transport of viral matrix proteins.