Effects of the acute phase response on the concentration and density distribution of plasma lipids and apolipoproteins.
Effects of the acute phase response on the concentration and density distribution of plasma lipids and apolipoproteins.
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DOI：
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发表时间：
1989
影响因子：
6.5
通讯作者：
V. Cabana;Joan N. Siegel;S. Sabesin
中科院分区：
文献类型：
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作者：
V. Cabana;Joan N. Siegel;S. Sabesin
在兔子模型中进行了纵向研究，以确定急性期反应 (APR) 期间血浆脂质和载脂蛋白浓度和密度分布的变化，其特征是 C 反应蛋白 (CRP) 和血清淀粉样蛋白 A (SAA) 水平升高。肌肉注射巴豆油12小时后，在高密度脂蛋白（HDL）中可检测到SAA。在反应高峰期（72 小时），HDL 下降，而 SAA 成为主要的 HDL 脱辅基蛋白，占较高密度级分中蛋白质的 80%。富含SAA的颗粒变得更密（HDL3的密度）但更大（HDL2的尺寸），电泳迁移率更慢，并且apoA-I、胆固醇、甘油三酯和磷脂被耗尽。 HDL-胆固醇减少并重新分配到其他部分，而apoA-I从循环中消失。在此期间，血浆甘油三酯增加了 6 至 10 倍，而血浆胆固醇和磷脂变化很小。 ApoB 增加 5 至 6 倍，而含 apoB 的颗粒密度增加，导致 IDL 升高，然后恢复期间 LDL 升高。 VLDL（d小于1.006 g/ml）增加并获得血浆甘油三酯、胆固醇、磷脂和apoB的30-40%。 VLDL 中的 SAA 也增加，而 apoE 则减少。
Longitudinal studies were carried out in the rabbit model to determine alterations in the concentration and density distribution of plasma lipids and apolipoproteins during the acute phase response (APR) characterized by elevated levels of C-reactive protein (CRP) and serum amyloid A (SAA). Twelve hr after the intramuscular injection of croton oil, SAA was detectable in high density lipoprotein (HDL). At the height of the response (72 hr), HDL decreased while SAA became the major HDL apoprotein, up to 80% of the proteins in the higher density fractions. The SAA-enriched particles became denser (density of HDL3) but larger (size of HDL2), had slower electrophoretic mobility, and were depleted in apoA-I, cholesterol, triglyceride, and phospholipid. HDL-cholesterol decreased and was redistributed to other fractions while apoA-I disappeared from the circulation. During this time plasma triglycerides increased 6- to 10-fold while plasma cholesterol and phospholipids showed minimal changes. ApoB increased 5- to 6-fold while the apoB-containing particles shifted to higher density resulting in elevated IDL and then LDL during recovery. VLDL (d less than 1.006 g/ml) increased and acquired 30-40% of the plasma triglycerides, cholesterol, phospholipid, and apoB. SAA also increased in VLDL while apoE decreased.