High-performance liquid chromatographic method for the simultaneous determination of the camptothecin derivative irinotecan hydrochloride, CPT-11, and its metabolites SN-38 and SN-38 glucuronide in rat plasma with a fully automated on-line solid-phase extraction system, PROSPEKT
High-performance liquid chromatographic method for the simultaneous determination of the camptothecin derivative irinotecan hydrochloride, CPT-11, and its metabolites SN-38 and SN-38 glucuronide in rat plasma with a fully automated on-line solid-phase extraction system, PROSPEKT
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DOI：
10.1016/s0378-4347(98)00554-4
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发表时间：
1999-03-19
期刊：
影响因子：
--
通讯作者：
Kaneda, N
中科院分区：
文献类型：
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作者：
Kurita, A;Kaneda, N
我们建立了高效液相色谱（HPLC）方法，采用全自动在线固相萃取系统PROSPEKT同时测定大鼠血浆中喜树碱（CPT）衍生物、盐酸伊立替康（CPT-11）及其代谢物7-乙基-10-羟基喜树碱（SN-38）和SN-38葡萄糖醛酸苷（SN-38G）。血浆样品用0.146 M H3PO4 预处理，灭活大鼠血浆中的羧酸酯酶和β-葡萄糖醛酸酶，加入内标溶液（0.146 M H3PO4，含1 μg/ml CPT）进行分析。该方法针对 CPT-11（5 至 25 000 ng/ml）、SN-38（5 至 2500 ng/ml）和 SN-38G（2.5 至 500 ng/ml）进行了验证。该方法能够在相对较短的时间内测定许多样品，并且样品制备简单。与传统测定方法相比，它还具有复杂样品制备自动化、同时测定三种化合物节省时间、直接测定SN-38G、测定所需血浆量少等四大优点。 (C) 1999 Elsevier Science B.V. 保留所有权利。
We established a high-performance liquid chromatography (HPLC) method for the simultaneous determination of the camptothecin (CPT) derivative, irinotecan hydrochloride (CPT-11) and its metabolites, 7-ethyl-10-hydroxycamptothecin (SN-38) and SN-38 glucuronide (SN-38G) in rat plasma with a fully automated on-line solid-phase extraction system, PROSPEKT. Plasma samples were pretreated with 0.146 M H3PO4 to inactivate carboxylesterase and beta-glucuronidase in rat plasma, and added with the internal standard solution (0.146 M H3PO4 containing 1 mu g/ml CPT) and then analyzed. The method was validated for CPT-11 (5 to 25 000 ng/ml), SN-38 (5 to 2500 ng/ml) and SN-38G (2.5 to 500 ng/ml). This method enabled the determination of many samples within a relatively short time with easy sample preparation. It also had four advantages compared with conventional determination methods, i.e. automation of a complicated sample preparation, time-saving by the simultaneous determination of three compounds, the direct determination of SN-38G, and the small amount of plasma required for the determination. (C) 1999 Elsevier Science B.V. All rights reserved.