Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.
Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species.
复制标题
lysS 和 lysU 的同源性，这两个大肠杆菌基因编码不同的赖氨酰-tRNA 合成酶种类。
作者：
Francoise Leveque;P. Plateau;P. Dessen;S. Blanquet
在大肠杆菌中，两种不同的赖氨酰-tRNA合成酶由两个基因编码：结构性的Lyss基因和热诱导的Lysu基因。这两个基因已经被分离和测序。它们的核苷酸和推导的氨基酸序列分别有79%和88%的同源性。密码子使用分析表明，Lyss产物的翻译效率高于Lysu产物。此外，Lyss序列与Herc的序列完全一致，Herc是prfB-Herc操纵子的一部分。与Gampel和Tzagoloff(1989，Proc.娜塔莉。阿卡德。SCI。美国86,6023-6027)，Lysu序列与位于frdA附近的开放阅读框不同，尽管这两个基因有很大的同源性。
In Escherichia coli, two distinct lysyl-tRNA synthetase species are encoded by two genes: the constitutive lysS gene and the thermoinducible lysU gene. These two genes have been isolated and sequenced. Their nucleotide and deduced amino acid sequences show 79% and 88% identity, respectively. Codon usage analysis indicates the lysS product being more efficiently translated than the lysU one. In addition, the lysS sequence exactly coincides with the sequence of herC, a gene which is part of the prfB-herC operon. In contrast to the recent proposal of Gampel and Tzagoloff (1989, Proc. Natl. Acad. Sci. USA 86, 6023-6027), the lysU sequence is distinct from the open reading frame located adjacent to frdA, although large homologies are shared by these two genes.