Cloning and characterization of human CIRP (cold-inducible RNA-binding protein) cDNA and chromosomal assignment of the gene
Cloning and characterization of human CIRP (cold-inducible RNA-binding protein) cDNA and chromosomal assignment of the gene
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DOI：
10.1016/s0378-1119(97)00530-1
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发表时间：
1997-12-19
期刊：
影响因子：
3.5
通讯作者：
Fujita, J
中科院分区：
文献类型：
--
作者：
Nishiyama, H;Higashitsuji, H;Fujita, J
冷胁迫诱导微生物合成几种蛋白质，这些蛋白质参与各种细胞过程，如转录、翻译和重组。最近，在轻度冷应激（32℃）下，啮齿动物细胞中发现了冷诱导rna结合蛋白（Cirp）。Cirp由一个n端rna结合域和一个c端富含gly结构域组成，在冷诱导的细胞增殖抑制中起重要作用。我们在此报道克隆了一个编码18 kda蛋白的cDNA，其氨基酸序列与小鼠Cirp的同源性为95.3%。利用荧光原位杂交技术将人CIRP基因定位到染色体19p13.3位点。CIRP mRNA在所有细胞系中组成性表达，包括K562、HepG2、NC65、HeLa、T24和NEC8细胞。在温度从37℃降至32℃后的12 h内，CIRP mRNA和蛋白水平均升高。这些结果表明，CIRP是人细胞中的冷休克蛋白。CIRP的鉴定可能为人类细胞冷反应的调控机制提供线索。(C) 1997爱思唯尔科学有限公司
Cold stress induces in microorganisms the synthesis of several proteins that are involved in various cellular processes such as transcription, translation and recombination. Recently, the cold-inducible RNA-binding protein (Cirp) was found to be induced in rodent cells by mild cold stress (32 degrees C). Cirp consists of an N-terminal RNA-binding domain and a C-terminal Gly-rich domain, and plays an essential role in cold-induced suppression of cell proliferation. We report here the cloning of a cDNA encoding an 18-kDa protein with 95.3% identity in an amino-acid sequence to that of mouse Cirp. The human CIRP gene has been mapped to the chromosomal locus 19p13.3 by fluorescence in-situ hybridization. CIRP mRNA is constitutively expressed in all cell lines examined, including K562, HepG2, NC65, HeLa, T24, and NEC8 cells. In all of them, the levels of CIRP mRNA and protein were increased within 12 h after a temperature down-shift from 37 degrees C to 32 degrees C. These results demonstrated that CIRP is a cold-shock protein in human cells. Identification of CIRP may provide a clue to the regulatory mechanisms of cold responses in human cells. (C) 1997 Elsevier Science B.V.