Effects of ambient ozone exposure on circulating extracellular vehicle microRNA levels in coronary artery disease patients
Effects of ambient ozone exposure on circulating extracellular vehicle microRNA levels in coronary artery disease patients
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DOI：
10.1080/15287394.2020.1762814
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发表时间：
2020-05-17
影响因子：
2.6
通讯作者：
Tong, Haiyan
中科院分区：
文献类型：
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作者：
Chen, Hao;Xu, Yunan;Tong, Haiyan
暴露于臭氧（O-3）和颗粒物（PM）等环境空气污染物与心血管发病率和死亡率增加有关，但其潜在的生物学机制尚未得到描述。新的证据表明，细胞外载体（EV）microRNAs（miRNAs）可能促进细胞与细胞和器官与器官的通讯，并在空气污染诱导的心血管效应中发挥作用。本研究旨在探讨空气污染物暴露与心血管疾病相关miRNA变化之间的关系。采用小组研究设计，14名患有冠状动脉疾病的参与者入选本研究。每个参与者有多达10次临床访视，收集他们的血浆样本并测量miRNA-21（miR-21），miR-126，miR-146，miR-150和miR-155的表达。根据温度、湿度和季节调整的混合效应模型用于研究miRNA水平与暴露于8小时O-3或24小时PM2.5之间的关联。结果表明，miR-150表达与O-3暴露在1-4天滞后和5天移动平均值呈正相关，而miR-155表达与O-3暴露在滞后0.在任何时间点，未发现miRNA表达与环境PM2.5之间存在显著关联。β受体阻滞剂和糖尿病药物的使用显著改变了O-3暴露与miR-150表达之间的相关性，其中非使用者之间的联系更为突出。总之，有证据表明暴露于环境O-3与EV miR-150和miR-155的循环水平之间存在相关性。这些发现指出了未来的研究方向，涉及miRNA介导的机制O-3诱导的心血管效应。
Exposure to ambient air pollutants such as ozone (O-3) and particulate matter (PM) is associated with increased cardiovascular morbidity and rate of mortality, but the underlying biological mechanisms have yet to be described. Emerging evidence shows that extracellular vehicle (EV) microRNAs (miRNAs) may facilitate cell-to-cell and organ-to-organ communications and play a role in the air pollution-induced cardiovascular effects. This study aims to explore the association between air pollutant exposure and miRNA changes related to cardiovascular diseases. Using a panel study design, 14 participants with coronary artery diseases were enrolled in this study. Each participant had up to 10 clinical visits and their plasma samples were collected and measured for expression of miRNA-21 (miR-21), miR-126, miR-146, miR-150, and miR-155. Mixed effects models adjusted for temperature, humidity, and season were used to examine the association between miRNA levels and exposure to 8-hr O-3 or 24-hr PM2.5 up to 4 days prior. Results demonstrated that miR-150 expression was positively associated with O-3 exposure at 1-4 days lag and 5day moving average while miR-155 expression tracked with O-3 exposure at lag 0. No significant association was found between miRNA expression and ambient PM2.5 at any time point. beta-blocker and diabetic medication usage significantly modified the correlation between O-3 exposure and miR-150 expression where the link was more prominent among non-users. In conclusion, evidence indicated an association between exposure to ambient O-3 and circulating levels of EV miR-150 and miR-155 was observed. These findings pointed to a future research direction involving miRNA-mediated mechanisms of O-3-induced cardiovascular effects.