Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile
Production of actin-specific ADP-ribosyltransferase (binary toxin) by strains of Clostridium difficile
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DOI：
10.1016/s0378-1097(00)00162-2
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发表时间：
2000-05-15
影响因子：
2.1
通讯作者：
Popoff, M
中科院分区：
文献类型：
--
作者：
Stubbs, S;Rupnik, M;Popoff, M
除了两种大的梭状芽胞杆菌细胞毒素(TcdA和TcdB)外，某些艰难梭菌株还能产生肌动蛋白特异性的ADP-核糖转移酶，或二元毒素。用聚合酶链式反应检测了该二元毒素的酶(CdtA)和结合(CdtB)基因，并对170株代表菌株进行了检测以评估该毒素的流行情况。免疫印迹和ADP-核糖基转移酶检测证实59例为阳性。TcdA和TcdB基因的聚合酶链式反应核型和毒素型(TcdA和TcdB基因的限制性片段长度多态性分析)与二元毒素基因的拥有相关。所有含有cdtA和cdtB的菌株都属于毒素可变的毒素型，并根据TcdA的存在与否描述了五类产毒素的菌株。Tcdb和二元毒素。结果显示，参考英国厌氧菌参考单位的艰难梭菌中，约6.4%的产毒菌株含有cdtA和cdtB基因。(C)2000年欧洲微生物学会联合会。爱思唯尔科学公司出版。版权所有。
In addition to the two large clostridial cytotoxins (TcdA and TcdB) certain strains of Clostridium difficile produce an actin-specific ADP-ribosyltransferase, or binary toxin. PCR reactions were developed to detect genes encoding the enzymatic (cdtA) and binding (cdtB) components of the binary toxin and 170 representative strains were tested to assess the prevalence of the toxin. Positive PCR results (n = 59) were confirmed by immunoblotting and ADP-ribosyltransferase assay. PCR ribotype and toxinotype (restriction fragment length polymorphism analysis of genes for TcdA and TcdB) correlated with possession of binary toxin genes. All strains with cdtA and cdtB belonged to toxin-variable toxinotypes and five toxin-producing groups of strains have been described according to the presence or absence of TcdA. TcdB and binary toxin. Result indicate that ca. 6.4% of toxigenic isolates of C. difficile referred to the Anaerobe Reference Unit from UK hospitals have cdtA and cdtB genes. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.