Visualization of Cortical Lamination Patterns with Magnetic Resonance Imaging
Visualization of Cortical Lamination Patterns with Magnetic Resonance Imaging
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DOI：
10.1093/cercor/bhr277
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发表时间：
2012-09-01
期刊：
影响因子：
3.7
通讯作者：
Assaf, Yaniv
中科院分区：
文献类型：
--
作者：
Barazany, Daniel;Assaf, Yaniv
在活体内成像皮质层层排列的能力是神经科学的圣杯之一。最近的研究已经使用高分辨率T-1/T-2磁共振成像(MRI)显示了体外和体内(感兴趣的小区域)的皮质层。在这项研究中，我们使用反转-恢复(IR)MRI来提高MRI对皮质结构的敏感度，并在活体中实现在人和大鼠身上的3D全脑表征各层。使用红外测量，我们计算了沿皮质的3D信号强度图，称为皮质图，以表征皮质亚结构。我们发现，沿着大脑皮层的多红外图像的聚类分析将其划分为至少6个层流区。为了验证我们的观察，我们将IR-MRI分析与组织学进行了比较，发现了一个对应关系，尽管这两个测量并不代表相似的数量。在纹状皮质(可视化的Gennari条纹)和额叶皮质上展示了该方法将皮质分割成多个层的能力。我们的结论是，所提出的方法可以作为研究和表征个体大脑皮层结构和组织的手段。
The ability to image the cortex laminar arrangements in vivo is one of the holy grails of neuroscience. Recent studies have visualized the cortical layers ex vivo and in vivo (on a small region of interest) using high-resolution T-1/T-2 magnetic resonance imaging (MRI). In this study, we used inversion-recovery (IR) MRI to increase the sensitivity of MRI toward cortical architecture and achieving whole-brain characterization of the layers, in vivo, in 3D on humans and rats. Using the IR measurements, we computed 3D signal intensity plots along the cortex termed corticograms to characterize cortical substructures. We found that cluster analyses of the multi-IR images along the cortex divides it into at least 6 laminar compartments. To validate our observations, we compared the IR-MRI analysis with histology and revealed a correspondence, although these 2 measures do not represent similar quantities. The abilities of the method to segment the cortex into layers were demonstrated on the striate cortex (visualizing the stripe of Gennari) and on the frontal cortex. We conclude that the presented methodology can serve as means to study and characterize individual cortical architecture and organization.