Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans
Droplet microfluidics for characterizing the neurotoxin-induced responses in individual Caenorhabditis elegans
复制标题
用于表征个体秀丽隐杆线虫神经毒素诱导反应的液滴微流体
DOI：
10.1039/c0lc00256a
复制
发表时间：
2010-01-01
期刊：
影响因子：
6.1
通讯作者：
Qin, Jianhua
中科院分区：
文献类型：
--
作者：
Shi, Weiwei;Wen, Hui;Qin, Jianhua
提出了一种基于液滴的微流控装置，该装置集成了一种新型的基于浮力的捕捉器阵列和锥形固定化通道阵列，用于连续表征神经毒素诱导的线虫个体的多重反应。建立的设备能够同时对线虫个体的运动和荧光成像分析进行评估。通过对神经毒素(6-羟基多巴胺，6-OHDA)引发的单个蠕虫的运动缺陷、神经元变性和氧化应激的药理学评价，证明了该装置的实用性。活体蠕虫暴露于6-OHDA可引起明显的运动障碍、多巴胺能神经元选择性变性和氧化应激增加，且呈剂量依赖关系。这些结果对于了解神经毒素引起神经毒性的机制具有重要意义，并将有助于筛选治疗神经退行性疾病的新疗法。该装置简单、稳定、易于操作，有可能在单个动物分辨率下以高通量的方式促进全动物分析和药物筛选。
A droplet-based microfluidic device integrated with a novel floatage-based trap array and a tapered immobilization channel array was presented for characterizing the neurotoxin-induced multiple responses in individual Caenorhabditis elegans (C. elegans) continuously. The established device enabled the evaluations of movement and fluorescence imaging analysis of individual C. elegans simultaneously. The utility of this device was demonstrated by the pharmacological evaluation of neurotoxin (6-hydroxydopamine, 6-OHDA) triggered mobility defects, neuron degeneration and oxidative stress in individual worms. Exposure of living worms to 6-OHDA could cause obvious mobility defects, selective degeneration of dopaminergic (DAergic) neurons, and increased oxidative stress in a dose dependent manner. These results are important towards the understanding of mechanisms leading to DAergic toxicity by neurotoxin and will be of benefit for the screening of new therapeutics for neurodegenerative diseases. This device was simple, stable and easy to operate, with the potential to facilitate whole-animal assays and drug screening in a high throughput manner at single animal resolution.