Lymphomagenesis and female-specific lethality in p53-deficient mice occur independently of E2f1.
Lymphomagenesis and female-specific lethality in p53-deficient mice occur independently of E2f1.
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p53 缺陷小鼠的淋巴瘤发生和雌性特异性致死率的发生与 E2f1 无关。
DOI：
10.1038/ncb0704-565
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发表时间：
2004
影响因子：
21.3
通讯作者：
Bronson,RoderickT
中科院分区：
文献类型：
--
作者：
Wloga,ElzbietaH;Criniti,Vittoria;Yamasaki,Lili;Bronson,RoderickT
图1缺失P53的动物的存活率不会因为失去E2F1而改变。构建了由A、B和C杂交(见补充信息)产生的p53缺陷动物的生存曲线，这些杂交是E2F1基因座上的野生型(n=56)或具有一个或两个E2F1等位基因突变的(n=119)或两个突变(n=51)。动物在其自然终点处死，或恰好在其自然终点之前被处死，以允许恢复组织用于肿瘤病变的组织病理学分析。P53缺陷组的平均生存年龄(t1/2)为：p53−/−E2F1+/+组(t1/2=170±7 d)，p53−/−E2F1+/−组(t1/2=153±5 d)，p53−/−E2F1+/−/−组(t1/2=158±7 d)。在任何p53缺失的队列中，平均存活年龄没有统计学差异(学生t检验，P>；0.05)。此外，对野生型对照动物(n=15)、E2F1+/−动物(n=27)和杂交A产生的E2F1基因缺陷动物(n=12)进行了365d的研究，期间仅有2只E2F1+/−动物和1只E2F1基因缺陷动物死亡。
Figure 1 Survival of p53-deficient animals is unchanged by loss of E2f1. Survival curves were constructed for p53-deficient animals generated from Crosses A, B and C (see Supplementary Information), which were either wild-type at the E2f1 locus (n= 56) or which had one (n= 119) or both (n= 51) E2f1 alleles mutated. Animals were sacrificed at, or just before, their natural endpoint to allow the recovery of tissues for the histopathological analysis of tumour lesions. The mean ages of survival (t1/2) for the p53-deficient cohorts are as follows: p53−/− E2f1+/+ animals (t1/2= 170±7 d); p53−/− E2f1+/− animals (t1/2= 153±5 d); p53−/− E2f1−/− animals (t1/2= 158±7 d). There was no statistical difference in the mean ages of survival for any of the p53-deficient cohorts (Student’s t-test, P> 0.05). In addition, wild-type control animals (n= 15), E2f1+/− animals (n= 27) and E2f1-deficient animals (n= 12) arising from Cross A were studied for 365 d, during which only two E2f1+/− animals and one E2f1-deficient animal died.